¥430.35
国内现货，预计发货时间2025年4月25日详情
等级
Molecular Biology
表单
buffered aqueous solution
运输
wet ice
储存温度
−20°C
1 of 4
此商品 | PHR1697 | 62143 | A8523 |
|---|---|---|---|
| antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria | antibiotic activity spectrum - | antibiotic activity spectrum Gram-positive bacteria | antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| assay 98.0-102.0% anhydrous basis (HPLC) | assay - | assay 96.0-102.0% (HPLC) | assay 95.0-102.0% anhydrous basis |
| form powder or crystals | form - | form powder or crystals | form powder |
| biological source synthetic | biological source - | biological source - | biological source - |
| Gene Information human ... KCNH1(3756) | Gene Information - | Gene Information - | Gene Information - |
一般描述
The Mammalian Amino-Terminal FLAG Stable Expression Kit provides the specialized FLAG® components needed for expression of a recombinant N-terminal FLAG fusion in mammalian cells. The pFLAG-CMV-3 and pFLAG-CMV-4 expression vectors encode the aminoglycoside phosphotransferase II gene (Neo) which confers resistance to aminoglycosides such as G418, allowing for selection of stable transfectants.
Vector Maps and Sequences
Vector Maps and Sequences
应用
The pFLAG-CMV-3 and pFLAG-CMV-4 Expression Vectors are suitable for transient or stable expression of secreted or cytoplasmic N-terminal FLAG® fusion proteins.
组分
- pFLAG-CMV-3a Expression Vector (E8770) 20 μg
- pFLAG-CMV-4a Expression Vector (E1775) 20 μg
- pFLAG-CMV-3-BAPa Control Plasmid (C3972) 20 μg
- pFLAG-CMV-4-BAPa Control Plasmid (C4722) 20 μg
- C-CMV-24 Reverse Sequencing Primer (P5475) 1 μg
- N-CMV-30 Forward Sequencing Primer (P5350) 1 μg
- ANTI-FLAG M2 Monoclonal Antibody (F3165) 200 μg
- ANTI-FLAG M5 Monoclonal Antibody (F4042) 200 μg
- ANTI-FLAG M2 Affinity Gel - Freezer Safe (A2220) 1 ml
- Met-FLAG-BAP Control Protein (P5975) 100 ug
- FLAG Peptide (F3290) 4 mg
原理
The FLAG® system is based on the FLAG marker octapeptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). The FLAG sequence is hydrophilic and the last 5 amino acids (Asp-Asp-Asp-Asp-Lys) represent the target sequence of the protease enterokinase. FLAG fusion proteins are created by cloning the coding sequence of a protein of interest adjacent to the FLAG coding sequence in a FLAG expression vector, followed by expression in the appropriate host cell type.
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
法规信息
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