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一般描述
甘油，通常也是指丙三醇，是一种3-碳糖醇，形成脂肪酸骨架。它是所有脂类合成的中心成分，是甘油三酯和磷脂的骨架，在细胞膜结构中起着重要作用。凭借低毒性，甘油被广泛用于制药、食品和化妆品配方，是通过酯交换法生产生物柴油的主要废物副产物。 除了试剂盒外，当需要进行少量反应时，可分别购买单独的试剂和甘油标准品。
应用
游离甘油测定试剂盒使用偶联酶反应测定游离内源性甘油，不包括初始脂肪酶水解。 甘油被5′-三磷酸腺苷（ATP）磷酸化，从而通过甘油激酶（GK）催化反应形成1-磷酸甘油（G-1-P）和5′-二磷酸腺苷（ADP）。 G-1-P随后被磷酸甘油氧化酶（GPO）氧化为磷酸二羟基丙酮（DAP）和过氧化氢(H2O2)。 过氧化物酶催化H2O2 与4-氨基安替比林(4-AAP)和n -乙基- n -(3-磺丙基)间苯胺钠(ESPA)偶联得到醌亚胺染料，其吸光度在540 nm处最大。540 nm处的吸光度增量与样品中的游离甘油浓度成正比。
游离甘油测定试剂盒用于测定体外培养的哺乳动物细胞释放到培养基中的甘油水平。
甘油三酯和自由甘油试剂盒可以用来定量测试甘油，总甘油三酯或自由甘油三酯。
适用性
适用于定量酶法测定血清或血浆中的甘油。
联系
除了试剂盒外，当需要进行少量反应时，可分别购买单独的试剂和甘油标准品。
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
含少量动物源组分生物产品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Hui Gao et al.
EBioMedicine, 30, 248-260 (2018-03-28)
Long non-coding RNAs (lncRNAs) belong to a recently discovered class of molecules proposed to regulate various cellular processes. Here, we systematically analyzed their expression in human subcutaneous white adipose tissue (WAT) and found that a limited set was differentially expressed
Sarah K Trottier et al.
Obesity (Silver Spring, Md.), 25(10), 1707-1715 (2017-08-08)
To compare the individual and combined effects of dairy and endurance exercise training in reducing weight gain and adiposity in a rodent model of diet-induced obesity. An 8-week feeding intervention of a high-fat, high-sugar diet was used to induce obesity
Elena Kosenko et al.
Journal of clinical medicine, 9(7) (2020-07-28)
The relationship between liver disease and neuropathology in hepatic encephalopathy is well known, but the genesis of encephalopathy in liver failure is yet to be elucidated. Conceptually, the main cause of hepatic encephalopathy is the accumulation of brain ammonia due
Hong Min Kim et al.
Nutrition (Burbank, Los Angeles County, Calif.), 55-56, 63-70 (2018-07-01)
Non-alcoholic fatty liver disease is characterized by high hepatic triacylglycerol contents, which is associated with endoplasmic reticulum (ER) stress and insulin resistance. Caffeic acid (CA) has antioxidant, immunomodulatory, and antiinflammatory effects. We investigated the effects of CA on hepatic steatosis
Geraldine Harriman et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(13), E1796-E1805 (2016-03-16)
Simultaneous inhibition of the acetyl-CoA carboxylase (ACC) isozymes ACC1 and ACC2 results in concomitant inhibition of fatty acid synthesis and stimulation of fatty acid oxidation and may favorably affect the morbidity and mortality associated with obesity, diabetes, and fatty liver
商品
Lipid Induced Insulin Resistance
脂肪组织中的胰岛素耐受性减少了循环游离脂肪酸的摄入，并增加了脂肪酶对储存甘油三酯的水解。了解与胰岛素耐受性相关的激素、细胞因子和脂肪因子。
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