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生物来源
synthetic
质量水平
偶联物
FITC conjugate
形式
powder
分子量
average mol wt 150,000
标记范围
0.003-0.025 mol FITC per mol glucose
颜色
faint yellow to orange
溶解性
H2O: 25 mg/mL, clear to slightly hazy, yellow-orange
储存温度
2-8°C
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一般描述
葡聚糖是一种脱水葡萄糖的聚合物。它由大约95％的α-D-(166)键组成。剩余的(163)连接依靠葡聚糖的支链。关于支链长度的矛盾数据意味着平均的支链长度小于三个葡萄糖单位。然而，其他方法表明存在大于50个葡萄糖单元的支链。已发现天然葡聚糖的分子量（MW）在900万至5亿的范围内。较低分子量的葡聚糖将表现出略微更少的支链化并具有更窄的分子量分布范围。分子量大于10,000的葡聚糖会表现的像是高度支链化的一样。随着分子量的增加，葡聚糖分子会获得更好的对称性。分子量为2,000至10,000的葡聚糖可表现出可膨胀线圈的特性。在分子量低于2,000时，葡聚糖更像是棒状。可通过以下一种或多种方法测量葡聚糖的分子量：低角度激光散射、尺寸排阻色谱、铜络合和蒽酮试剂比色法还原糖测定和粘度。
应用
FITC-葡聚糖作为荧光通量示踪剂化合物，可被用于心血管、微循环、灌注、细胞单层和细胞膜通透性研究，支持血流、膜损伤、血管引流和肾脏消除等过程的测量。 小的FITC-葡聚糖也可用于测量内吞作用和细胞连接通透性等过程。荧光素-葡聚糖，150kDa可助力于血脑屏障（BBB）的完整性评估以及进行荧光微淋巴造影（FML）。 荧光素-葡聚糖150可用作微循环血管通量示踪剂。
FITC-葡聚糖可广泛用于利用微荧光法的微循环和细胞渗透性研究。FITC-葡聚糖已被用于研究植物细胞壁的孔隙率以及毛细管的渗透性。血浆蛋白已证明不会与FITC-葡聚糖结合。
用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。
制备说明
Sigma通常以25 mg/ml或更高的浓度测试FITC葡聚糖在水中的溶解度。溶液应避光保存。在体内，FITC-葡聚糖可稳定超过24小时。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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相关内容
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
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