检测方案
≥98% (TLC)
形式
powder
颜色
off-white to yellow
mp
153-157 °C (lit.)
溶解性
acetone: 50 mg/mL
储存温度
room temp
SMILES字符串
O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
InChI
1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
InChI key
ZFKJVJIDPQDDFY-UHFFFAOYSA-N
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一般描述
荧光素是一种杂环二酮，与伯胺、氨基酸、肽和蛋白质反应形成荧光产物。过量的试剂及其降解产物不发荧光。含有蛋白质加氟胺的溶液的荧光强度与存在的游离胺基团量相当。这是荧光蛋白测定的基本原理。氟胺用作鉴定制药工业所用各类化合物的衍生剂，如奥司他韦、青霉胺、磺酰胺残基、赖诺普利、甲氨蝶呤、维巴替林和加巴喷丁。
应用
荧光素用作染色剂，通过共聚焦扫描激光显微镜（CSLM）定位小米淀粉表面的颗粒蛋白以及小米粉中的蛋白质。这一物质还用于对氨基苯甲酸光度测定。
非荧光试剂，在温和条件下易与氨基酸和肽中的伯胺反应形成稳定的高荧光化合物。由于水解，背景低。可用于氨基酸、蛋白质和蛋白水解酶的荧光测定。在蛋白质序列分析中有效阻断新产生的氨基末端。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
P C Hollman et al.
The American journal of clinical nutrition, 62(6), 1276-1282 (1995-12-01)
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S Udenfriend et al.
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This account highlights some of our recent activities focused on developing microfluidic technologies for application in high-throughput and high-information content chemical and biological analysis. Specifically, we discuss the use of continuous and segmented flow microfluidics for artificial membrane formation, the
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