重组
expressed in baculovirus infected Sf21 cells
质量水平
方案
>97% (SDS-PAGE)
表单
lyophilized powder
效能
0.03-0.1 μg per mL ED50 (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)
分子量
55 kDa (glycosylated form)
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
UniProt登记号
储存温度
−20°C
基因信息
mouse ... Fas(14102)
一般描述
Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.
应用
Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).
生化/生理作用
Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.
分析说明
Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Davidson D Ateh et al.
Biomaterials, 32(33), 8538-8547 (2011-08-10)
The CD95/CD95L receptor-ligand system is mainly recognised in the induction of apoptosis. However, it has also been shown that CD95L is over-expressed in many cancer types where it modulates immune-evasion and together with its receptor CD95 promotes tumour growth. Here
B A Treviño-Talavera et al.
Genetics and molecular research : GMR, 13(3), 4831-4838 (2014-07-26)
Primary Sjögren's syndrome (pSS) is a chronic systemic autoimmune disease characterized by lymphocytic infiltration of exocrine glands. Soluble Fas receptor (sFas) has been suggested as a Fas-mediated apoptosis blocker that could impair clonal deletion in infiltrated autoreactive cells. The FAS
J Cheng et al.
Science (New York, N.Y.), 263(5154), 1759-1762 (1994-03-25)
Fas is an apoptosis-signaling receptor molecule on the surface of a number of cell types. Molecular cloning and nucleotide sequence analysis revealed a human Fas messenger RNA variant capable of encoding a soluble Fas molecule lacking the transmembrane domain because
A Oehm et al.
The Journal of biological chemistry, 267(15), 10709-10715 (1992-05-25)
The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 was previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. Cross-linking of the
N Itoh et al.
Cell, 66(2), 233-243 (1991-07-26)
Mouse anti-Fas monoclonal antibody has a cytolytic activity on human cells that express the antigen. Complementary DNAs encoding the cell surface antigen Fas were isolated from a cDNA library of human T cell lymphoma KT-3 cells. The nucleotide sequence of
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