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安全信息

F8435

Sigma-Aldrich

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌

lyophilized powder, recombinant, expressed in E. coli

别名:

N-糖苷酶F, 糖苷酶F 来源于脑膜炎脓毒性黄杆菌, 糖苷酶F 来源于脑膜脓毒性金黄杆菌, 肽N-糖苷酶

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50 UNITS
¥2,325.51
300 UNITS
¥11,674.00

¥2,325.51

国内现货，预计发货时间2025年4月23日详情

新价格，新优惠！
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50 UNITS
¥2,325.51
300 UNITS
¥11,674.00

About This Item

CAS号:
83534-39-8
EC 号:
3.5.1.52 (BRENDA, IUBMB)
MDL编号:
MFCD00131222
UNSPSC代码:
12352204
NACRES:
NA.54

¥2,325.51

国内现货，预计发货时间2025年4月23日详情

新价格，新优惠！

重组

expressed in E. coli

质量水平

200

偶联物

(N-linked)

等级

Proteomics Grade

表单

lyophilized powder

比活

≥1,000 U/mg

保质期

≥1 weeks at 2‑8 °C (for reconstituted solution)
≥1 yr at -20 °C

分子量

~36 kDa

运输

wet ice

储存温度

−20°C

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此商品
G0535G1549P7367
糖苷酶F 来源于脑膜脓毒性伊丽莎白菌 lyophilized powder, recombinant, expressed in E. coli

F8435

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌

糖肽酶 A 来源于杏仁 buffered aqueous glycerol solution, ≥0.05 unit/mL

G0535

糖肽酶 A 来源于杏仁

PNGase F脑膜炎沙门氏菌 ready-to-use solution, recombinant, expressed in E. coli

G1549

PNGase F脑膜炎沙门氏菌

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌 BioReagent, ≥95% (SDS-PAGE)

P7367

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌

grade

Proteomics Grade

grade

-

grade

Proteomics Grade

grade

for proteomics

form

lyophilized powder

form

buffered aqueous glycerol solution

form

ready-to-use solution

form

powder

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

recombinant

-

mol wt

~36 kDa

mol wt

-

mol wt

~36 kDa

mol wt

~36 kDa

shelf life

≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C

shelf life

-

shelf life

≥1 yr at -20 °C

shelf life

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml), ≥1 yr at 2‑8 °C, Solution is stable for at least 3 freeze-thaw cycles

应用

用于使蛋白质去糖基化。
高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。

生化/生理作用

从糖蛋白切割整个聚糖，前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。

包装

在一项调查他汀类药物对p-糖蛋白的双重影响及其对人神经母细胞瘤细胞中阿霉素细胞毒性影响的研究中，PNGase F已被用于P-糖蛋白的去糖基化。[1]它在一项研究中的定量免疫印迹分析前被用于处理纯化的蛋白，小鼠视锥紫外线（MUV）色素[2]它已被用于对N-连接的糖蛋白进行糖基化。高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。

单位定义

一个单元将在37℃、pH7.5下通过SDS-PAGE监测，在1分钟内催化1纳摩尔变性核糖核酸酶B释放N-连接的寡糖。 PNGase F活性的一个 Sigma 单位等于1 IUB毫单位。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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    分析证书(COA)

    Lot/Batch Number
    0000432197
    0000432197
    0000405902
    0000405901
    0000405901

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    Methods in molecular biology (Clifton, N.J.), 2271, 155-167 (2021-04-29)
    O-glycosylation is a difficult posttranslational modification to analyze. O-glycans are labile and often cluster making their analysis by LC-MS very challenging. OpeRATOR is an O-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues. This
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    T H Plummer et al.
    The Journal of biological chemistry, 259(17), 10700-10704 (1984-09-10)
    Endo-beta-N-acetylglucosaminidase F preparations from Flavobacterium meningosepticum have been found to contain peptide:N-glycosidase activity. Only the second activity, designated as peptide:N-glycosidase F, readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide, as shown by the isolation of the corresponding
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