应用
用于使蛋白质去糖基化。
高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。
生化/生理作用
从糖蛋白切割整个聚糖，前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。
包装
在一项调查他汀类药物对p-糖蛋白的双重影响及其对人神经母细胞瘤细胞中阿霉素细胞毒性影响的研究中，PNGase F已被用于P-糖蛋白的去糖基化。它在一项研究中的定量免疫印迹分析前被用于处理纯化的蛋白，小鼠视锥紫外线（MUV）色素它已被用于对N-连接的糖蛋白进行糖基化。高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。
单位定义
一个单元将在37℃、pH7.5下通过SDS-PAGE监测，在1分钟内催化1纳摩尔变性核糖核酸酶B释放N-连接的寡糖。 PNGase F活性的一个 Sigma 单位等于1 IUB毫单位。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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