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应用
可用于通过 O′Brien, W., Anal. Biochem., FB76, 423 (1976) 介绍的方法进行焦磷酸盐测定。
生化/生理作用
Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.
其他说明
这两种焦磷酸依赖性果糖-6-磷酸激酶的区别在于它们对生理活化剂果糖 2,6-二磷酸 [Fru (2,6)-P2]的反应不同。据报道，来自费氏丙酸杆菌的酶 (F8381) 不受 Fru (2,6)-P2 影响。而来自马铃薯块茎的酶 (F2258) 则可被 Fru (2,6)-P2 激活（在 5.5nM Fru (2,6)-P2 条件下，半最大活化马铃薯块茎酶）。
单位定义
在 pH 7.4 和 30°C 下，一单位每分钟可将 1.0μmol 的焦磷酸盐和果糖-6-磷酸转化为果糖-1,6-二磷酸和无机磷酸盐。
外形
含咪唑盐和稳定剂
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
B L Bertagnolli et al.
Biochemistry, 23(18), 4101-4108 (1984-08-28)
Inorganic pyrophosphate dependent D-fructose-6-phosphate 1-phosphotransferase from Propionibacterium freudenreichii was purified to apparent homogeneity by the criterion of silver staining on sodium dodecyl sulfate (SDS) gels. In the direction of phosphorylation of fructose 6-phosphate (F6P), an intersecting initial velocity pattern is
Daniel L Machell et al.
Chembiochem : a European journal of chemical biology, 23(18), e202200325-e202200325 (2022-07-26)
Detection of pyrophosphate is important in quantifying enzyme activity, particularly adenylation domain activity during non-ribosomal peptide synthesis. The previous development of an enzyme coupled PPi /NADH assay allowed the measurement of such activity in an online fashion using commercially available
Corrigendum: Online Pyrophosphate Assay for Analyzing Adenylation Domains of Nonribosomal Peptide Synthetases.
Tiia Kittilä et al.
Chembiochem : a European journal of chemical biology, 23(18), e202200381-e202200381 (2022-07-28)
J Suzuki et al.
Genetics and molecular research : GMR, 2(4), 376-382 (2004-03-11)
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have
M E Theodorou et al.
Planta, 213(1), 147-157 (2001-08-29)
A major problem in defining the physiological role of pyrophosphate:fructose 6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90) is the 1,000-fold discrepancy between the apparent affinity of PFP for its activator, fructose 2,6-bisphosphate (Fru-2,6-P2), determined under optimum conditions in vitro and the estimated
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