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生物来源
mouse
质量水平
偶联物
FITC conjugate
抗体形式
purified immunoglobulin
克隆
UPC-10, monoclonal
表单
buffered aqueous solution
储存条件
protect from light
技术
flow cytometry: suitable
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
IgG2a (immunoglobulin G 2a) is one of the subclasses of rat immunoglobulins, which also include IgG1, IgG2b and IgG2c.
特异性
Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.
应用
IgG2a FITC Isotype Control from murine myeloma has been used in dual-colour staining and in cell staining for flow cytometry.
生化/生理作用
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
制备说明
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
含少量动物源组分生物产品
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1 * 1501 Is Regulated by Vitamin D
Ramagopalan SV, et al.
PLoS Genetics, 5(2), e1000369-e1000369 (2009)
Three classes and four (sub) classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c
Bazin H, et al.
European Journal of Immunology, 4, 44-48 (1974)
T Nakamura et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(9), 5389-5394 (1998-06-06)
Activation of hepatic stellate (Ito) cells is a final common pathway of liver fibrosis. The findings presented in this paper indicate that expression of Na+/Ca2+ exchanger (NCX) emerges in rat hepatic stellate cells after activation in vitro during primary culture
Assessment of Contractility of Purified Smooth Muscle Cells Derived from Embryonic Stem Cells
Sinha S, et al.
Stem Cells, 24(7), 1678-1688 (2006)
J Schultz et al.
Cancer research, 60(23), 6663-6669 (2000-12-16)
Single-chain Fv antibody fragments from the CD20-specific murine monoclonal antibody B9E9 were genetically engineered as streptavidin fusions [single-chain Fv-streptavidin (scFvSA) fusion protein] for use in pretargeted radioimmunotherapy. The scFvSA constructs were expressed as soluble, tetrameric species in the periplasm of
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