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Merck
CN

F6522

Sigma-Aldrich

IgG2a−FITC Isotype Control from murine myeloma

clone UPC-10, purified immunoglobulin, buffered aqueous solution

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别名:
IgG2a−Fluorescein isothiocyanate
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

mouse

质量水平

偶联物

FITC conjugate

抗体形式

purified immunoglobulin

克隆

UPC-10, monoclonal

形式

buffered aqueous solution

储存条件

protect from light

技术

flow cytometry: suitable

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

一般描述

IgG2a (immunoglobulin G 2a) is one of the subclasses of rat immunoglobulins, which also include IgG1, IgG2b and IgG2c.

特异性

Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.

应用

IgG2a FITC Isotype Control from murine myeloma has been used in dual-colour staining and in cell staining for flow cytometry.

生化/生理作用

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

制备说明

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

含少量动物源组分生物产品
常规特殊物品

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Stem Cells, 24(7), 1678-1688 (2006)
T Nakamura et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(9), 5389-5394 (1998-06-06)
Activation of hepatic stellate (Ito) cells is a final common pathway of liver fibrosis. The findings presented in this paper indicate that expression of Na+/Ca2+ exchanger (NCX) emerges in rat hepatic stellate cells after activation in vitro during primary culture
Three classes and four (sub) classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c
Bazin H, et al.
European Journal of Immunology, 4, 44-48 (1974)
Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1 * 1501 Is Regulated by Vitamin D
Ramagopalan SV, et al.
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Single-chain Fv antibody fragments from the CD20-specific murine monoclonal antibody B9E9 were genetically engineered as streptavidin fusions [single-chain Fv-streptavidin (scFvSA) fusion protein] for use in pretargeted radioimmunotherapy. The scFvSA constructs were expressed as soluble, tetrameric species in the periplasm of

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