跳转至内容
Merck
CN
所有图片(1)

主要文件

查看所有文档

安全信息

F5766

Sigma-Aldrich

Monoclonal Anti-Folic Acid antibody produced in mouse

clone VP-52, ascites fluid

登录查看公司和协议定价
所有图片(1)

About This Item

MDL编号:
MFCD00162295
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

VP-52, monoclonal

包含

15 mM sodium azide

技术

indirect ELISA: 1:500

同位素/亚型

IgG2b

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

相关类别

一般描述

Monoclonal Anti-Folic Acid (mouse IgG2b isotype) is derived from the hybridoma produced from the fusion of mouse myeloma cells and splenocytes of an immunized mouse. The normal range for folate in serum is 3-13 ng/ml.

特异性

Monoclonal Anti-Folic Acid antibody binds specifically to folate and detects an epitope present on both folic acid and its biologically active analog 5-methyltetrahydrofolic acid (5MTHFA). The product reacts with free folate, folate conjugated to a carrier such as KLH or BSA, and folic acid in human plasma or serum bound to the endogenous Folate Binder. The antibody does not cross-react with tetrahydrofolic acid, folinic acid or dihydrofolic acid.

免疫原

5-Methyltetrahydrofolic acid (5MTHFA) conjugated to KLH

应用

Monoclonal Anti-Folic Acid antibody is suitable for use in western blot (diluted 1:1000).

生化/生理作用

Folic acid (folate) is a vitamin that regulates one-carbon transfer during the conversion of homocysteine to methionine. Methionine is a precursor of S-adenosylmethionine, which is the main methyl donor for methylation reactions. Thus, folic acid is essential for regulation of DNA methylations and for epigenetic modifications that modulate gene expression. Folate deficiency has been associated with a wide range of disorders including colorectal cancers, depression, neural tube defects and cerebral dysfunctions
Folic acid is essential for normal growth of mammalian cells. Folic acid deficiencies are common in pregnant women, alcoholics, those whose diets do not include raw fruits and vegetables, and people with structural or functional damage to the small intestine. Vitamin B12 and folic acid are metabolically interrelated.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品？  

试试我们的产品选型工具.

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000120826
0000120827
0000112963
0000108346
0000108347

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What immunogen was used to generate Product No. F5766, Monoclonal Anti-Folic Acid antibody?

    The immunogen used was 5-Methyltetrahydrofolic acid (sometimes called 5MTH folic acid) conjugated to keyhole limpet hemocyanin (KLH).

  7. What is the name of the antibody clone for Product No. F5766, Monoclonal Anti-Folic Acid antibody?

    The clone is VP-52.

  8. What is the concentration of Product No. F5766, Monoclonal Anti-Folic Acid antibody?

    The concentration of the antibody is lot specific and can be found on the certificate of analysis. (It is typically in the 1-2 mg/mL range).

  9. What is the isotype of Product No. F5766, Monoclonal Anti-Folic Acid antibody?

    This antibody is mouse IgG2B.

  10. Will Product No. F5766, Monoclonal Anti-Folic Acid antibody, work in Western blotting?

    The use of this antibody for western blotting has been reported in one study on Pseudomonas and tumor cells. See: Leamon, C.P., et al., Cytotoxicity of Folate-Pseudomonas Exotoxin Conjugates Toward Tumor Cells. J. Biol. Chem., 268(33), 24847-24854 (1993). The antibody was used at a 1 to 1000 dilution in Tris buffered saline (TBS) for 1 hour at 23°C.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Periconceptional folate deficiency and implications in neural tube defects.
Safi, J., et al.
Journal of Pregnancy, doi: 10-doi: 10 (2012)
Giuseppe Destito et al.
Chemistry & biology, 14(10), 1152-1162 (2007-10-27)
Cowpea mosaic virus (CPMV) is a well-characterized nanoparticle that has been used for a variety of nanobiotechnology applications. CPMV interacts with several mammalian cell lines and tissues in vivo. To overcome natural CPMV targeting and redirect CPMV particles to cells
Keith Hyland et al.
Journal of inherited metabolic disease, 33(5), 563-570 (2010-07-30)
Cerebral folate deficiency (CFD) is defined as any neurological syndrome associated with a low cerebrospinal fluid (CSF) concentration of 5-methyltetrahydrofolate (5MTHF) in the presence of normal peripheral folate status. CFD has a wide clinical presentation, with reported signs and symptoms
Young-In Kim
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 13(4), 511-519 (2004-04-07)
Epidemiological, clinical, and animal studies collectively indicate that dietary folate intake and blood folate levels are inversely associated with colorectal cancer risk. Folate plays an essential role in one-carbon transfer involving remethylation of homocysteine to methionine, which is a precursor
Folic acid: A positive influence on periodontal tissues during health and disease
George JP, et al.
INTERNATIONAL JOURNAL OF ADVANCED MULTIDISCIPLINARY RESEARCH, 2(3), 145-145 (2013)
查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门