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一般描述
FITC（异硫氰酸荧光素）是一种荧光染料，可吸收紫外线或蓝光，使分子激发并发出可见的黄绿色光。 该发射在去除引起激发的光后停止。 当反应物之一是细胞、组织或其他生物结构的一部分时，荧光染料标记可快速、准确地定位抗原-抗体相互作用。 FITC是免疫荧光技术中常用的抗体标记物，因为FITC与蛋白质的结合相对容易，并且通常不会破坏标记蛋白的生物活性。 FITC作为半抗原被广泛用于标记不同的蛋白质。 FITC抗体用于鉴定FITC标记的蛋白质，并作为模型来研究抗体对明确定义的半抗原的反应机制。
特异性
小鼠单克隆克隆FL-D6抗FITC抗体将与游离或偶联FITC反应。该抗体未与结合的或游离的TRITC（异硫氰酸四甲基罗丹明）反应。
该抗体可与游离的和结合的异硫氰酸荧光素（FITC）反应。
免疫原
FITC-BSA偶联物
应用
小鼠单克隆克隆FL-D6抗FITC抗体可用于检测FITC，并作为通用指示剂，用于将FITC与其他免疫化学试剂桥接。 可用于ELISA、竞争ELISA和免疫荧光技术。 FITC抗FITC系统已用于放大免疫荧光检测中的信号，并通过亲和色谱法将结合物与游离示踪剂分离。 该抗体也可用于分离在其表面上具有FITC标记配体的细胞。
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WGK
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闪点(°F)
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闪点(°C)
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法规信息
常规特殊物品
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