Monoclonal Anti-IgD−FITC antibody produced in mouse

Recognizes the delta chain of human IgD and is not reactive with heavy chains from human IgG1, IgG2, IgG3, IgG4, IgM, IgA, or kappa and lambda light chains.

human IgD

Monoclonal Anti-IgD-FITC antibody is suitable for flow cytometry at a concentration of 10μL using 1×10⁶ cells.

IgD is the major antigen receptor isotype coexpressed with IgM on the surface of human peripheral B cells. It is associated with early antibody response. It occupies approximately 0.25% of total immunoglobulins. In serum, it has shown that the catabolism of IgD is decreased at high concentrations. IgD molecule comprises of a long “hinge” region (between Fab and Fc) including three constant region domains. This makes the molecule very susceptible to proteolytic degradation with production of Fab and Fc fragments. It also helps to maintain the molecule flexibility that enhance antigen binding capacity. IgD is a potent inducer of tumor necrosis factor (TNF), Interlukin 1β(IL1β), and Interlukin IL1RN (IL1RN). It also accelerates the release of IL6, IL10 from peripheral blood mononuclear cells. Its increased level in serum causes hyper-IgD and periodic fever syndrome (HIDS).

IgD is expressed on peripheral B cells and is used as a pan-B cell marker.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

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