应用
SIGMAFAST Fast Red TR/萘酚AS-MX片剂已用于:
- 在器官切片中进行碱性磷酸酶染色
- 用于免疫组化研究中软骨样球体的染色
- 用于人腭扁桃体切片原位mRNA杂交研究中杂交信号的检测
开发用作免疫化学中碱性磷酸酶活性检测的沉淀底物。主要用途是免疫组织学、免疫印迹或斑点印迹。SIGMAFAST™ Fast Red TR / Naphthol AS-MX 片剂含有0.6 mM 左旋咪唑,可阻断内源性碱性磷酸酶活性。SIGMAFAST™ Fast Red TR/Naphthol AS-MX 片剂无需额外的步骤或缓冲液来制备活性底物溶液。
重悬
将每片溶于1mL 去离子水中,得到即用型缓冲溶液。
法律信息
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
警示用语:
Danger
危险声明
危险分类
Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。
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