生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M5, monoclonal
形式
buffered aqueous solution
纯化方式
using Protein A
种属反应性
all
浓度
2-5 mg/mL
技术
western blot (chemiluminescent): 10 μg/mL
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
一般描述
单克隆 ANTI-FLAG ® M5 是一种小鼠抗体，可与 N-末端 Met-FLAG 融合蛋白结合。可用于检测哺乳动物和果蝇细胞中表达的 N-末端 Met-FLAG 融合蛋白。
纯化方法-蛋白A
纯化方法-蛋白A
免疫原
FLAG；肽序列 DYKDDDDK
应用
小鼠生产的单克隆抗FLAG®M5抗体已被用于免疫印迹和免疫荧光。
只有当 FLAG 肽位于甲硫氨酸前面的氨基末端时，才能与其结合。结合不依赖于 Ca2+。可用于检测哺乳动物细胞粗提物中细胞质表达的 Met-FLAG 融合蛋白，但不推荐用于在大肠杆菌中表达的融合蛋白。
外形
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
制备说明
用 Tris 缓冲盐水 (TBS) 将抗体稀释至 10 mg/mL：0.05 M Tris，0.15 M NaCl，pH 7.4。
其他说明
抗体不具有钙依赖性。
法律信息
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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