F4042
单克隆抗 FLAG ® M5 小鼠抗
clone M5, purified immunoglobulin, buffered aqueous solution
别名:
抗 ddddk, 抗 dykddddk
登录 查看组织和合同定价。
选择尺寸
关于此项目
Conjugate:
unconjugated
Clone:
M5, monoclonal
Application:
western blot (chemiluminescent)
Species reactivity:
all
Citations:
186
Technique(s):
western blot (chemiluminescent): 10 μg/mL
产品名称
单克隆抗 FLAG ® M5 小鼠抗, clone M5, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M5, monoclonal
form
buffered aqueous solution
purified by
using Protein A
species reactivity
all
concentration
2-5 mg/mL
technique(s)
western blot (chemiluminescent): 10 μg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
只有当 FLAG 肽位于甲硫氨酸前面的氨基末端时，才能与其结合。结合不依赖于 Ca2+。可用于检测哺乳动物细胞粗提物中细胞质表达的 Met-FLAG 融合蛋白，但不推荐用于在大肠杆菌中表达的融合蛋白。
小鼠生产的单克隆抗FLAG®M5抗体已被用于免疫印迹和免疫荧光。
General description
单克隆 ANTI-FLAG ® M5 是一种小鼠抗体，可与 N-末端 Met-FLAG 融合蛋白结合。可用于检测哺乳动物和果蝇细胞中表达的 N-末端 Met-FLAG 融合蛋白。
纯化方法-蛋白A
纯化方法-蛋白A
Immunogen
FLAG；肽序列 DYKDDDDK
Other Notes
抗体不具有钙依赖性。
Physical form
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
Preparation Note
用 Tris 缓冲盐水 (TBS) 将抗体稀释至 10 mg/mL：0.05 M Tris，0.15 M NaCl，pH 7.4。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism
Furman C, et al.
The Journal of Biological Chemistry, 277(10), 7962-7969 (2002)
Bader Almuzzaini et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(8), 2860-2873 (2016-04-30)
Actin and nuclear myosin 1 (NM1) are regulators of transcription and chromatin organization. Using a genome-wide approach, we report here that β-actin binds intergenic and genic regions across the mammalian genome, associated with both protein-coding and rRNA genes. Within the
CCAAT/enhancer-binding protein beta regulates the repression of type II collagen expression during the differentiation from proliferative to hypertrophic chondrocytes
Ushijima T, et al.
The Journal of Biological Chemistry, 289(5), 2852-2863 (2014)
Franz Oswald et al.
Nucleic acids research, 44(10), 4703-4720 (2016-02-26)
The transcriptional shift from repression to activation of target genes is crucial for the fidelity of Notch responses through incompletely understood mechanisms that likely involve chromatin-based control. To activate silenced genes, repressive chromatin marks are removed and active marks must
Chih-Chao Liang et al.
Nature communications, 7, 12124-12124 (2016-07-14)
The Fanconi anaemia (FA) pathway is important for the repair of DNA interstrand crosslinks (ICL). The FANCD2-FANCI complex is central to the pathway, and localizes to ICLs dependent on its monoubiquitination. It has remained elusive whether the complex is recruited
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持