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Merck
CN

F3418

Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse

clone C-11, purified immunoglobulin, buffered aqueous solution

别名:

Monoclonal Anti-Pan Cytokeratin

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203

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产品名称

Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse, clone C-11, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

C-11, monoclonal

form

buffered aqueous solution

species reactivity

bovine, mouse, frog, human, kangaroo rat, rat

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:50 using PtK2 cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human placenta
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

bovine ... Krt1(100301161)
human ... KRT1(3848), KRT13(3858), KRT13(3858), KRT18(3875), KRT18(3875), KRT4(3851), KRT4(3851), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856)
mouse ... Krt1(16678), Krt10(16661), Krt10(16661), Krt13(16663), Krt13(16663), Krt18(16668), Krt18(16668), Krt4(16682), Krt4(16682), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt1-18(706059), Krt1-18(706059), Krt10(450225), Krt10(450225), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626)

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Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Cytokeratin, pan−FITC antibody has been used in immunocytochemistry and has also been used to visualize lung epithelial cells.

Biochem/physiol Actions

Cytokeratins helps in subtyping several kinds of epithelial differentiation or for narrowing possible sites of origin for metastatic carcinomas. Cytokeratins may play an important role in transhepatic transport and canalicular secretion.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cytokeratins are the intermediate filaments of endocrine cells. These proteins belong to the intermediate filament (10 nm) superfamily of cytoskeletal proteins. Cytokeratins are the largest and most complex group of intermediate filaments. The family consists of at least 30 proteins with molecular weights ranging from 40kDa to 68kDa.

Immunogen

keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000480987
0000480987
0000166455
0000226332
0000187125

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Olivia Jerczynski et al.
PloS one, 11(10), e0163876-e0163876 (2016-10-04)
Dicer1 is an endoribonuclease involved in the biogenesis of functional molecules such as microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs). These small non-coding RNAs are important regulators of post-transcriptional gene expression and participate in the control of male fertility.
Autocrine GABA signaling distinctively regulates phenotypic activation of mouse pulmonary macrophages
Januzi L, et al.
Cellular Immunology, 332(2), 7-23 (2018)
Potential biological role of transforming growth factor-beta in human congenital kidney malformations
Yang Su P, et al.
The American Journal of Pathology, 157(5), 1633-1647 (2000)
Matthew L Tomlinson et al.
Frontiers in nutrition, 4, 61-61 (2018-01-13)
Flavonoids are a diverse group of plant secondary metabolites, known to reduce inflammatory bowel disease symptoms. How they achieve this is largely unknown. Our study focuses on the gut epithelium as it receives high topological doses of dietary constituents, maintains
C J Morrow et al.
Annals of oncology : official journal of the European Society for Medical Oncology, 27(6), 1155-1160 (2016-03-26)
Over the past decade, numerous reports describe the generation and increasing utility of non-small-cell lung cancer (NSCLC) patient-derived xenografts (PDX) from tissue biopsies. While PDX have proven useful for genetic profiling and preclinical drug testing, the requirement of a tissue
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Data Sheet - F3418

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