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安全信息

F3046

Sigma-Aldrich

单克隆抗肌动蛋白抗体

clone AC-40, purified immunoglobulin, buffered aqueous solution

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别名:
Monoclonal Anti-Actin
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

200

偶联物

FITC conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

AC-40, monoclonal

形式

buffered aqueous solution

分子量

antigen 42 kDa

种属反应性

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

技术

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

同位素/亚型

IgG2a

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... ACTA1(58) , ACTA1(58) , ACTA1(58) , ACTA2(59) , ACTA2(59) , ACTA2(59) , ACTB(60) , ACTB(60) , ACTB(60) , ACTC1(70) , ACTC1(70) , ACTC1(70) , ACTG1(71) , ACTG1(71) , ACTG1(71) , ACTG2(72) , ACTG2(72) , ACTG2(72)
mouse ... Acta1(11459) , Acta1(11459) , Acta1(11459) , Acta2(11475) , Acta2(11475) , Acta2(11475) , Actb(11461) , Actb(11461) , Actb(11461) , Actc1(11464) , Actc1(11464) , Actc1(11464) , Actg1(11465) , Actg1(11465) , Actg1(11465) , Actg2(11468) , Actg2(11468) , Actg2(11468)
rat ... Acta1(29437) , Acta1(29437) , Acta1(29437) , Acta2(81633) , Acta2(81633) , Acta2(81633) , Actb(81822) , Actb(81822) , Actb(81822) , Actc1(29275) , Actc1(29275) , Actc1(29275) , Actg1(287876) , Actg1(287876) , Actg1(287876) , Actg2(25365) , Actg2(25365) , Actg2(25365)

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一般描述

该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

免疫原

与多抗原肽（MAP）骨架连接的合成肌动蛋白C末端肽

应用

已使用肌动蛋白单克隆抗体作为一抗，对MDCK细胞裂解物进行蛋白质免疫印迹分析。
肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析，以确保等量蛋白质上样。
Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.

生化/生理作用

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

其他说明

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

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J L Lessard
Cell motility and the cytoskeleton, 10(3), 349-362 (1988-01-01)
Two IgG1, kappa monoclonal antibodies (Mab) against actin have been obtained from a fusion in which chicken gizzard actin was used as the immunogen. One Mab, designated B4, shows a preferential reactivity toward enteric smooth muscle actin but also cross-reacts
K Sutoh et al.
Biochemistry, 25(20), 6186-6192 (1986-10-07)
An antibody was raised against the N-terminal 18 residues of rabbit skeletal muscle actin. By the use of this antibody as the N-terminal probe of actin and the fluorescent label at Cys-374 as its C-terminal probe, binding sites of depactin
Kimberly A Moore et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 232(2), 268-281 (2004-12-23)
Local alterations in the mechanical compliance of the basement membrane that alter the level of isometric tension in the cell have been postulated to influence tissue morphogenesis. To explore whether cell tension contributes to tissue pattern formation in vivo, we
I M Herman
Current opinion in cell biology, 5(1), 48-55 (1993-02-01)
The actin supergene family encodes a number of structurally related, but perhaps functionally distinct, protein isoforms that regulate contractile potential in muscle tissues and help to control the shape as well as the motility of non-muscle cells. In spite of
J S Drew et al.
The American journal of physiology, 260(6 Pt 1), C1332-C1340 (1991-06-01)
Smooth muscle cells contain both smooth muscle-specific and cytoplasmic actin isoforms. We tested the hypothesis that these two classes of isoactins are segregated into separate "contractile" vs. "cytoskeletal" populations of thin filaments. Isolated thin filaments from adult swine stomach were
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