类型
Type 70
质量水平
形式
powder
分子量
~70,000
颜色
white
溶解性
H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow
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一般描述
Ficoll® PM70是一种高分子量支化多糖聚合物，公认不含任何离子化基团。这种蔗糖聚合物由蔗糖与环氧氯丙烷共聚形成。
应用
Ficoll® PM 70已用于：
- 用作比较研究中的大分子，评估其在玻璃化溶液中的效果
- 用作卵母细胞玻璃化过程的玻璃化溶液补充剂
- 用作人肺成纤维细胞培养的低血清培养基成分，促进形成拥挤环境
生化/生理作用
Ficoll 常用作玻璃化溶液的大分子成分。由于其密集的分支，Ficolls也可用作理想的近球形分子拥挤剂。
在细胞体积信号转导和蛋白质重折叠研究中，FicollTM PM 70用作大分子拥挤剂。这一物质可用于组织工程和大分子构象研究，开发、评估和使用大分子拥挤 (MMC) 系统和配置。 Ficoll PM-70还可用于玻璃化研究。
法律信息
Ficoll is a registered trademark of Cytiva
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
M B Burg
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 10(5-6), 251-256 (2000-12-23)
The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occur in cells, but that these are
C M Checura et al.
Theriogenology, 67(5), 919-930 (2006-12-19)
This study was designed to evaluate vitrification procedures for in vitro matured bovine oocytes for efficient blastocyst production after warming, IVF and culture. A second goal was to replace serum as the macromolecular component of the vitrification solution, without compromising
Y Georgalis et al.
Journal of colloid and interface science, 386(1), 141-147 (2012-08-23)
Ficoll PM70 is an important branched polysaccharide polymer with a variety of applications in biology and biophysics. The accepted picture for the aqueous solutions of this polymer is that of a polydisperse nearly spherical suspension with a molecular weight of
M Al-Habori
The international journal of biochemistry & cell biology, 33(9), 844-864 (2001-07-20)
Macromolecular crowding has been proposed as a mechanism by means of which a cell can sense relatively small changes in volume or, more accurately, the concentration of intracellular solutes. According to the macromolecular theory, the kinetics and equilibria of enzymes
Xumeng Ge et al.
PloS one, 6(12), e28707-e28707 (2011-12-17)
Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect the efficiency of protein
实验方案
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。
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