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重组
expressed in baculovirus infected Sf9 cells
质量水平
表单
buffered aqueous solution
分子量
57 kDa
浓度
≥2,000 unit/mL
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... FURIN(5045)
相关类别
应用
弗林蛋白酶能够裂解多种蛋白质的前体,包括生长因子、血清蛋白(包括凝血和补体系统的蛋白酶)、基质金属蛋白酶、受体、病毒包膜糖蛋白和细菌外毒素,通常在共有序列 Arg-Xaa-(Lys/Arg)-Arg 标记的位点。
生化/生理作用
弗林蛋白酶是一种二元内切蛋白酶,定位于高尔基体。分子量为 52.7 kDa。在哺乳动物细胞的分泌和内吞途径中负责许多前体蛋白的蛋白水解成熟。Furin 在其配对的碱性氨基酸加工位点切割前体蛋白。弗林蛋白酶的一些底物包括 von Willebrand 因子、转化生长因子 β 1 前体,前 β-分泌酶和前甲状旁腺激素 。
弗林蛋白酶是一种二元内切蛋白酶,定位于高尔基体。在哺乳动物细胞的分泌和内吞途径中负责许多前体蛋白的蛋白水解成熟。
单位定义
一 个单位的定义为25°C下、6小时内总反应体积25μl中裂解 25 μg MBP-FN- 副肌球蛋白-ΔSal 底物达到95%完成度所需的酶量。
外形
溶于 10 mM MES,pH 7.0,25°C,1 mM CaCl2,50% 甘油中。
制备说明
分离自 携带截短人弗林蛋白酶的重组杆状病毒感染的草地贪夜蛾 (Sf9) 细胞
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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