形式
ammonium sulfate suspension
质量水平
比活
≥300 units/mg protein (biuret)
异质活性
malic dehydrogenase ≤0.1%
储存温度
2-8°C
一般描述
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.
Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.
应用
Fumarase from porcine heart has been used:
- as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
- for immobilized enzyme based stability assay
- in fumarate hydratase assays in human lung carcinoma cells (A549)
Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.
生化/生理作用
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.
单位定义
One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.
外形
Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol
制备说明
Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Identification of the catalytic mechanism and estimation of kinetic parameters for fumarase
Mescam M, et al.
The Journal of Biological Chemistry, jbc-M110 (2011)
Fumarase: a paradigm of dual targeting and dual localized functions
Yogev O, et al.
FEBS Journal, 278(22), 4230-4242 (2011)
Study of an enzyme membrane reactor with immobilized fumarase for production of L-malic acid
Giorno L, et al.
Biotechnology and Bioengineering, 72(1), 77-84 (2001)
Fumarase Deficiency: A Safe and Potentially Disease Modifying Effect of High Fat/Low Carbohydrate Diet
Ryder B, et al.
JIMD Reports, 40(3), 77?83-77?83 (2018)
Discovering targets of non-enzymatic acylation by thioester reactivity profiling
Kulkarni RA, et al.
Cell Chemical Biology, 24(2), 231-242 (2017)
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
实验方案
Enzymatic Assay of Fumarase
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