推荐产品
生物来源
bovine fetus
质量水平
无菌性
sterile-filtered
产品线
FBS Select
组成
Bovine IgG, <5 μg/mL
Hemoglobin, ≤20 mg/dL
来源
USA origin
储存条件
avoid repeated freeze/thaw cycles
protect from light
技术
cell culture | mammalian: suitable
杂质
≤10 EU/mL endotoxin
pH值（酸碱度）
6.8-8.1
渗透压
260-330 mOsm/kg H2O
运输
dry ice
储存温度
−20°C
一般描述
Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
包装
Packaged in sterilized, graduated plastic bottles and sealed with a tamper indicator. Bottles are immediately frozen and stored at -10 to -40 C.
制备说明
The fetal blood is aseptically collected and allowed to clot under controlled conditions. After centrifugation, the raw (unfiltered) serum is decanted from the clot, pooled and immediately frozen.
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
分析说明
Endotoxin and hemoglobin tested
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
实验方案
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
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