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Merck
CN

F1269

Sigma-Aldrich

Anti-Avidin–FITC antibody, Mouse monoclonal

clone WC19.10, purified from hybridoma cell culture

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别名:
Monoclonal Anti-Avidin
UNSPSC代码:
12352203
NACRES:
NA.43

生物来源

mouse

质量水平

偶联物

FITC conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

WC19.10, monoclonal

形式

buffered aqueous solution

储存条件

protect from light

技术

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect immunofluorescence: suitable

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

相关类别

一般描述

Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Avidin is a glycoprotein (68 kDa) composed of four identical polypeptide chains isolated from egg whites.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

特异性

FITC conjugated monoclonal anti-avidin may be used for single or double immunofluorescence labeling techniques and for modification of non-fluorescent to fluorescent reactions.

免疫原

Purified egg white avidin.

应用

Anti-Avidin-FITC antibody, Mouse monoclonal has been used in indirect immunohistology.

生化/生理作用

Avidin has a high binding affinity for the vitamin biotin. This strong interaction has been exploited in the design of immunoassays and immunohistologic staining techniques to serve as a basis for identifying antigen antibody interactions

外形

Solution supplied in 0.01 M phosphate buffered saline, pH 8.0, containing 1% BSA and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

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Adsorption of avidin on microfabricated surfaces for protein biochip applications
Bashir R, et al.
Biotechnology and Bioengineering, 73(4), 324-328 (2001)
John T Berg et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(8), 1101-1106 (2004-07-20)
Carbonic anhydrase (CA) is ubiquitously expressed and plays a pivotal role in acid-base balance, ion transport, and gas exchange. Limited observations by others, derived from functional, pharmacological, and histochemical studies, suggest that CA is present in vascular smooth muscle and
Xuefeng Wang et al.
Applied optics, 47(15), 2779-2789 (2008-05-22)
We perform simultaneous interferometric and fluorescent detection of molecular protein layers on a BioCD. The 488 nm excitation wavelength of fluorescein also provides the interferometric detection channel that operates in a common-path in-line configuration in the condition of phase quadrature

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