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相关类别
应用
荧光素透明质酸已用于透明质酸 (ha) 和葡聚糖表面涂层膜 和图案化表面的荧光成像。在共聚焦荧光显微镜中也用于定位胶原/HA 复合凝胶中的透明质酸。
生化/生理作用
利用与荧光素胺偶联的透明质酸大分子 (HA) 通过荧光相关光谱法 (FCS) 通过测量荧光素标记的 HA 片段的猝灭来筛选透明质酸酶活性。它结合在源自人肿瘤的恶性间皮瘤的细胞表面上。
用于与人类肿瘤来源的恶性间皮瘤细胞表面结合。
其他说明
来自鸡冠或通过发酵。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Hyaluronan receptors are expressed on human malignant mesothelioma cells but not on normal mesothelial cells
Asplund T and Heldin P
Cancer research, 54(16), 4516-4523 (1994)
T Asplund et al.
Cancer research, 54(16), 4516-4523 (1994-08-15)
Hyaluronan-binding sites were demonstrated on the cell surface of three malignant mesothelioma cell lines derived from human tumors using either [3H]hyaluronan or fluorescein-tagged hyaluronan. No hyaluronan-binding activity was observed on normal human mesothelial cells. The absence of hyaluronan receptors on
Rheology and confocal reflectance microscopy as probes of mechanical properties and structure during collagen and collagen/hyaluronan self-assembly
Yang Yl and Kaufman LJ
Biophysical Journal, 96(4), 1566-1585 (2009)
Human macrophage adhesion on polysaccharide patterned surfaces
Tsai IY, et al.
Soft Matter, 7(7), 3599-3606 (2011)
Rafal Fudala et al.
Journal of photochemistry and photobiology. B, Biology, 106, 69-73 (2011-11-16)
In this report we propose a lifetime-based sensing (LBS) for the detection of hyaluronidase (HA-ase). First, we heavily label hyaluronan macromolecules (HAs) with fluorescein amine. The fluorescein labeled HA (HA-Fl) has a weak fluorescence and short fluorescence lifetime due to
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