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主要文件

安全信息

F1141

Sigma-Aldrich

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

别名:

纤连蛋白 溶液 来源于人类成纤维细胞

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About This Item

EC 号:
MDL编号:
UNSPSC代码:
12352202
eCl@ss:
42020141
NACRES:
NA.75

产品名称

纤连蛋白牛血浆, solution, sterile-filtered, BioReagent, suitable for cell culture

生物来源

bovine plasma

质量水平

无菌性

sterile-filtered

产品线

BioReagent

表单

solution

包装

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

技术

cell culture | mammalian: suitable

表面覆盖度

1‑5 μg/cm2

UniProt登记号

结合特异性

Peptide Source: Collagen

Peptide Source: Fibrinogen

运输

wet ice

储存温度

2-8°C

基因信息

bovine ... FN1(280794)

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一般描述

纤连蛋白是一种普遍存在的细胞外基质蛋白。它是一种镶嵌蛋白,由三种的重复模块共有氨基酸序列组成。纤连蛋白属于一类高分子量糖蛋白。细胞纤连蛋白由成纤维细胞表达,血浆纤连蛋白通常由肝细胞释放。

应用

纤连蛋白(FN)牛血浆可用于:
  • 纤连蛋白和吸附分析(ELISA(酶联免疫吸附测定))
  • 跨孔迁移
  • 纤连蛋白测定

生化/生理作用

纤连蛋白参与细胞粘附、细胞骨架组织、致癌性转化、细胞迁移、吞噬、止血和胚胎分化。

分析说明

采用免疫电泳法评估蛋白质的均一性。

其他说明

产品F1141,牛血浆纤连蛋白是pH 7.5的0.05 M TBS溶液。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

监管及禁止进口产品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I choose which fibronectin product to use to coat culture surfaces for cell growth?

    The species source for the fibronectin used when coating culture surfaces is not important.  It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.

  4. How long can I store the fibronectin solution?

    The product is good for at least a year at 2-8 °C if kept sterile.

  5. How long can I store fibronectin coated plates, etc?

    Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.

  6. What is the procedure for coating culture surfaces with fibronectin?

    To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume.  2. Allow to air dry for at least 45 minutes at room temperature.  Excess fibronectin may be removed by aspiration, but this is not necessary.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 and p42/44MAPK dependent mechanisms
Fang W B, et al.
The Journal of biological chemistry, jbc-M112 (2012)
FIBRONECTIN AUTOANTIBODIES
Autoantibodies, 221-251 (2007)
Enhanced fibronectin adsorption on carbon nanotube/poly (carbonate) urethane: independent role of surface nano-roughness and associated surface energy
Khang D, et al.
Biomaterials, 28(32), 4756-4768 (2007)
Tests to predict preeclampsia
Chesley's Hypertensive Disorders in Pregnancy, 221-251 (2015)
Role of subnano-, nano-and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells
Khang D, et al.
Biomaterials, 33(26), 5997-6007 (2012)

商品

纤连蛋白(FN)是由肝细胞产生的多功能细胞外基质糖蛋白,以接近微摩尔的浓度循环,并与局部产生的细胞FN一起在细胞表面组装成胞外基质原纤维。

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

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实验方案

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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