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Merck
CN

F1141

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

别名:

纤连蛋白 溶液 来源于人类成纤维细胞

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关于此项目

UNSPSC Code:
12352202
eCl@ss:
42020141
NACRES:
NA.75
EC Number:
289-149-2
MDL number:
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产品名称

纤连蛋白牛血浆, solution, sterile-filtered, BioReagent, suitable for cell culture

biological source

bovine plasma

sterility

sterile-filtered

product line

BioReagent

form

solution

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

UniProt accession no.

binding specificity

Peptide Source: Collagen

Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

2-8°C

Quality Level

Gene Information

bovine ... FN1(280794)

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Analysis Note

采用免疫电泳法评估蛋白质的均一性。

Application

纤连蛋白(FN)牛血浆可用于:
  • 纤连蛋白和吸附分析(ELISA(酶联免疫吸附测定))
  • 跨孔迁移
  • 纤连蛋白测定

Biochem/physiol Actions

纤连蛋白参与细胞粘附、细胞骨架组织、致癌性转化、细胞迁移、吞噬、止血和胚胎分化。

General description

纤连蛋白是一种普遍存在的细胞外基质蛋白。它是一种镶嵌蛋白,由三种的重复模块共有氨基酸序列组成。纤连蛋白属于一类高分子量糖蛋白。细胞纤连蛋白由成纤维细胞表达,血浆纤连蛋白通常由肝细胞释放。

Other Notes

产品F1141,牛血浆纤连蛋白是pH 7.5的0.05 M TBS溶液。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I choose which fibronectin product to use to coat culture surfaces for cell growth?

    The species source for the fibronectin used when coating culture surfaces is not important.  It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.

  4. How long can I store the fibronectin solution?

    The product is good for at least a year at 2-8 °C if kept sterile.

  5. How long can I store fibronectin coated plates, etc?

    Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.

  6. What is the procedure for coating culture surfaces with fibronectin?

    To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume.  2. Allow to air dry for at least 45 minutes at room temperature.  Excess fibronectin may be removed by aspiration, but this is not necessary.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Biomaterials, 33(26), 5997-6007 (2012)

商品

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

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实验方案

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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