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安全信息

F0804

Sigma-Aldrich

胎牛血清,研究级

non-USA origin, sterile-filtered, suitable for cell culture

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别名:
胎牛血清, FBS, FCS, 血清
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.71

生物来源

bovine fetus

质量水平

400
500

无菌性

sterile-filtered

产品线

FBS Classic

组成

hemoglobin (tested)

来源

non-USA origin

存货情况

available only in EU

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested
mycoplasma, tested
virus (BVD, IBR, PI3), tested negative

运输

dry ice

储存温度

−20°C

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一般描述

胎牛血清(FBS)是一种常用的增补剂,主要来源于未出生的小牛。它含有营养素、 维生素、激素、转运蛋白以及贴附、扩散和生长因子。

应用

研究级胎牛血清已被用作以下培养基的增补剂:
  • 用于培养人脐静脉内皮细胞(HUVEC)的内皮细胞培养基(ECMed)
  • 用于重新悬浮人脂肪组织来源的基质细胞(ASC)的Dulbecco′s 改良Eagle′s 培养基(DMEM)
  • 用于 体外卵子成熟的组织培养基199 (TCM199)
  • 用于培养Madin-Darby犬肾(MDCK) II 细胞的最低必需培养基(MEM)Earle′s 培养基。

生化/生理作用

胎牛血清(FBS)主要用于器官、细胞和组织培养。 FBS是所有真核细胞培养基的首选增补剂。它为大多数类型细胞的增殖和活力提供养分。

分析说明

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

分析证书(COA)

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Kayleigh M van Megen et al.
Frontiers in immunology, 10, 694-694 (2019-04-20)
Mesenchymal stromal cells (MSCs) are inherently immunomodulatory through production of inhibiting soluble factors and expression of immunosuppressive cell surface markers. We tested whether activated MSCs qualify for the induction of antigen-specific immune regulation. Bone marrow derived human MSCs were activated
Simona Ranallo et al.
Nature communications, 8, 15150-15150 (2017-05-10)
A wide range of molecular devices with nanoscale dimensions have been recently designed to perform a variety of functions in response to specific molecular inputs. Only limited examples, however, utilize antibodies as regulatory inputs. In response to this, here we
Sara Kaliman et al.
Frontiers in physiology, 7, 551-551 (2016-12-10)
It is well accepted that cells in the tissue can be regarded as tiles tessellating space. A number of approaches were developed to find an appropriate mathematical description of such cell tiling. A particularly useful approach is the so called
J van der Valk et al.
Toxicology in vitro : an international journal published in association with BIBRA, 18(1), 1-12 (2003-11-25)
Fetal bovine serum (FBS) is a common supplement to in vitro culture media. A workshop was organized to discuss whether or not fetuses might suffer when blood is withdrawn, and to discuss serum replacement methods. When bovine fetuses are exposed
Jan van der Valk
Science (New York, N.Y.), 375(6577), 143-144 (2022-01-14)
Ethical and possible reproducibility issues arise when using fetal bovine serum in cell culture media.

商品

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

回顾使用胎牛(或小牛)血清进行细胞培养的基础知识,以及采购和法规如何影响细胞测定所用血清的价格。

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