F0628
铁氧化还原蛋白-NADP + 还原酶 来源于菠菜
lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase
别名:
铁氧还蛋白-NADP+ 氧化还原酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥15 units/mg solid
form
lyophilized powder
specific activity
≥15 units/mg solid
foreign activity
NADH diaphorase activity ≤10 units/mg solid
storage temp.
−20°C
Quality Level
General description
铁氧还蛋白-NADP+ 还原酶是可溶于铁氧还蛋白的配偶子，与PSI的摩尔比为0.9和3。
Application
Ferredoxin-NADP + 还原酶用于 体外 蓝藻类囊体膜中脂肪酸的铁氧化还原蛋白依赖性去饱和作用。也用于调节甘油醛-3-磷酸脱氢酶。
从 Spinacia oleracea（菠菜）中提取的铁氧还蛋白-NADP+ 还原酶用于试验潜在蛋白配偶子对3-磷酸甘油醛脱氢酶（GAPDH）−CP12制剂活性的影响。它也用于标准测定条件，测定藻蓝素：铁氧还蛋白氧化还原酶（PcyA）酶。
Biochem/physiol Actions
铁氧还蛋白-NADP + 还原酶在光合作用过程中催化还原铁氧还蛋白可逆转化为氧化铁氧还蛋白 。铁氧还蛋白-NADP (H) 还原酶组成了一个亲水性含 FAD 单体酶家族，该家族将 NADPH 或低潜力单电子供体传递给质体、线粒体和细菌中基于氧化还原的代谢。
铁氧还蛋白-NADP + 还原酶在光合作用过程中催化还原铁氧还蛋白可逆转化为氧化铁氧还蛋白 。铁氧还蛋白-NADP (H) 还原酶组成了一个亲水性含 FAD 单体酶家族，该家族将 NADPH 或低潜力单电子供体传递给质体、线粒体和细菌中基于氧化还原的代谢。
Other Notes
在菠菜铁氧还蛋白和 NADP 存在的情况下，在 25℃、ph7.5 的条件下，一个单位将每分钟还原 1.0 毫摩尔的细胞色素 C。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Néstor Carrillo et al.
European journal of biochemistry, 270(9), 1900-1915 (2003-04-24)
Ferredoxin (flavodoxin)-NADP(H) reductases (FNR) are ubiquitous flavoenzymes that deliver NADPH or low potential one-electron donors (ferredoxin, flavodoxin) to redox-based metabolisms in plastids, mitochondria and bacteria. The plant-type reductase is also the basic prototype for one of the major families of
Malika Mekhalfi et al.
The New phytologist, 203(2), 414-423 (2014-05-07)
Diatoms are a widespread and ecologically important group of heterokont algae that contribute c. 20% to global productivity. Previous work has shown that regulation of their key Calvin cycle enzymes differs from that of the Plantae, and that in crude
A Conserved Histidine-Aspartate Pair Is Required for Exovinyl Reduction of Biliverdin by a Cyanobacterial Phycocyanobilin:Ferredoxin Oxidoreductase
Tu SL, et al.
The Journal of Biological Chemistry, 281(6), 3127-3136 (2006)
Min Seok Cho et al.
Journal of microbiology (Seoul, Korea), 50(3), 496-501 (2012-07-04)
The Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis is the causal agent of canker disease in tomato. Because it is very important to control newly introduced inoculum sources from commercial materials, the specific detection of this pathogen in seeds and seedlings
Ferredoxin-NADP+ Reductase KINETICS OF ELECTRON TRANSFER, TRANSIENT INTERMEDIATES, AND CATALYTIC ACTIVITIES STUDIED BY FLASH-ABSORPTION SPECTROSCOPY WITH ISOLATED PHOTOSYSTEM I AND FERREDOXIN
Cassan N, et al.
The Journal of Biological Chemistry, 280(28), 25960-25972 (2005)
实验方案
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
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