纯化方式
(Single-spin negative chromotography)
(Time: 45 minutes or less)
特点
Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For the purificiation of genomic DNA from human or animal tissue)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
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sustainability
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环保替代产品分类
相关类别
一般描述
- Isolation of DNA in under an hour with minimal sample handling
- Better purity, leading to improved performance in PCR and other applications
- Significant reduction in plastic and hazardous chemical waste
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
应用
The initial lysis step takes place under physiological conditions where the enzyme activity of proteases is generally increased. SmartLyse® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This eliminates the requirement for extended, overnight incubations, which are required in many conventional protocols.
Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is equal or better than silica-based methods depending on sample type. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
特点和优势
储存及稳定性
其他说明
法律信息
警示用语:
Danger
危险分类
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
10 - Combustible liquids
WGK
WGK 3
法规信息
商品
GenElute™-E DNA purification kits ensure accurate DNA quantitation and downstream enzymatic processes.
相关内容
GenElute™-E DNA和RNA一次离心纯化技术可从哺乳动物细胞、血液、组织及植物组织样本中快速提取并纯化DNA和RNA。
GenElute™-E Single Spin DNA and RNA purification technology enables rapid DNA and RNA extraction and purification from mammalian cells, blood, tissue, and plant tissue samples.
Animation showing technology principle of GenElute™-E single spin negative chromatography nucleic acid purification kits
有关GenElute™-E DNA和RNA单次离心纯化和负选色谱常见问题的解答
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