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Merck
CN

E9640

Sigma-Aldrich

Endothelial cell growth factor

100 ×, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC代码:
12352202

生物来源

bovine pituitary glands

质量水平

无菌性

sterile-filtered

包装

pkg of 1 L
pkg of 10 mL
pkg of 5 mL

储存条件

avoid repeated freeze/thaw cycles

浓度

100 ×

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

运输

dry ice

储存温度

−70°C

应用

Endothelial Cell Growth Factor (ECGF) has been optimized for the culture of Human Umbilical Vein Endothelial Cells (HUVEC). ECGF is optimized for the mitogenic proliferation of HUVEC cells when supplemented to CS-C Medium. ECGF should be supplemented to CS-C medium at a concentration 1 ml per 100 ml of culture medium. ECGF is prepared in DPBS (without calcium and magnesium) supplemented with porcine heparin and HBGF-1. For best results it is recommended that this product be used in conjugation with CS-C Medium for Endothelial Cell Lines (Product Nos. C 1431 or C 1556), Endothelial Attachment Factor (Product No. E 9765), Trypsin/EDTA Solution for Endothelial Cell Cultures (Product No. T 4299) and Trypsin Inhibitor Solution (Product No. T 6414).

制备说明

5 mg/ml each porcine heparin and HBGF-1 in DPBS without calcium and magnesium

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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