产品名称
弹性蛋白酶 来源于猪胰腺, lyophilized powder, suitable for cell culture
biological source
Porcine
form
lyophilized powder
specific activity
≥4 units/mg protein
mol wt
25.9 kDa
packaging
pkg of 1 μmol
technique(s)
cell culture | mammalian: suitable
pH
8
suitability
suitable for
foreign activity
Trypsin ≤50 BAEE units/mg protein
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
来自猪胰腺的弹性蛋白酶用于以下应用:
- 细胞培养技术
- ELISA 技术
- 用于测定弹性蛋白酶抑制活性
Biochem/physiol Actions
弹性蛋白酶水解弹性蛋白(弹性纤维的特定蛋白质)并消化血红蛋白、酪蛋白和纤维蛋白。
降低弹性蛋白的酶,弹性蛋白是参与拉伸过程的组织的基质成分。它是一种具有广泛特异性的丝氨酸蛋白酶。它切割小疏水性氨基酸(如 Ile、Gly、Ala、Ser、Val 和 Leu)的羧基侧的蛋白质。该酶还水解酰胺和酯,例如 N-苯甲酰基-L-丙氨酸甲酯。
General description
弹性蛋白酶是 240 个氨基酸残基的单一多肽链,含有四个二硫键。分子量约为 25.9kDa。该酶被合成为无活性的酶原,原弹性蛋白酶,其通过胰蛋白酶在 N-末端的有限蛋白水解而转化为活性形式。
Other Notes
一个单元将在 25℃ 下,每分钟,pH8.0,水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。
Packaging
包装尺寸基于蛋白质含量
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
动植物源性产品
此项目有
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商品
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
实验方案
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
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