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一般描述
弹性蛋白酶是 240 个氨基酸残基的单一多肽链，含有四个二硫键。分子量约为 25.9kDa。该酶被合成为无活性的酶原，原弹性蛋白酶，其通过胰蛋白酶在 N-末端的有限蛋白水解而转化为活性形式。
应用
来自猪胰腺的弹性蛋白酶用于以下应用：
- 细胞培养技术
- ELISA 技术
- 用于测定弹性蛋白酶抑制活性
生化/生理作用
弹性蛋白酶水解弹性蛋白（弹性纤维的特定蛋白质）并消化血红蛋白、酪蛋白和纤维蛋白。
降低弹性蛋白的酶，弹性蛋白是参与拉伸过程的组织的基质成分。它是一种具有广泛特异性的丝氨酸蛋白酶。它切割小疏水性氨基酸（如 Ile、Gly、Ala、Ser、Val 和 Leu）的羧基侧的蛋白质。该酶还水解酰胺和酯，例如 N-苯甲酰基-L-丙氨酸甲酯。
包装
包装尺寸基于蛋白质含量
单位定义
一个单元将在 25℃ 下，每分钟，pH8.0，水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。
应用
底物
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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商品
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
实验方案
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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