Anti-eIF4ENIF1 (N-terminal) 兔抗

Eukaryotic translation initiation factor 4E nuclear import factor 1 (eIF4ENIF1) comprises two leucine-rich nuclear export signals and a bipartite nuclear localization signal. eIF4ENIF1 gene is mapped to human chromosome 22q12.2 and is expressed in the human ovary.

Anti-eIF4ENIF1(N-terminal) specifically recognizes human eIF4ENIF1.

synthetic peptide corresponding amino acids 1-19 of human eIF4ENIF1, conjugated to KLH via a C-terminal added cysteine residue. This sequence is specific to human.

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit is suitable for the following applications:

• Immunoprecipitation at a concentration of 5-10μL using HeLa cell lysates
• Indirect immunofluorescence at a concentration of 5-10μg/mL using paraformaldehyde-fixed HEK-293T cells
• Western blotting at a concentration of 1-2μg/mL using HeLa cell lysates

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein encoded by the EIF4ENIF1 gene in humans. It is a nucleocytoplasmic shuttling protein that mediates the import of eIF4E into the nucleus. 4E-transporter (4E-T) is one of several proteins that bind the mRNA 5′cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. Its interaction with eIF4E is a priming event in inducing messenger ribonucleoprotein rearrangement and transition from translation to decay. Eukaryotic initiation factor 4E plays an important role in controlling cell growth. 4E-T is essential for the assembly of processing bodies and its overexpression triggers the movement of eIF4E into the processing bodies.

eIF4ENIF1 (also known as 4E-T or 4E-transporter) colocalizes with other mRNA binding/processing proteins to processing (P)-bodies. Its interaction with eIF4E is essential for targeting of mRNAs to P-bodies. eIF4ENIF1 competes with eIF4G for binding to eIF4E and preventing the formation of the eIF4F complex thus inhibits translation. Together with 4E-BPs, it participates in cell response to prolonged hypoxia, sequestering eIF4E, and affecting gene expression. A nonsense mutation in the eIF4ENIF1 gene is implicated in the Primary Ovarian Insufficiency.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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