E6412
纤维二糖水解酶I from Hypocrea jecorina
100 U/mg, recombinant, expressed in corn
别名:
Cel7A, 纤维二糖水解酶, 纤维素酶
产品名称
纤维二糖水解酶I from Hypocrea jecorina, 100 U/mg, recombinant, expressed in corn
recombinant
expressed in corn
form
liquid
specific activity
100 U/mg
greener alternative product characteristics
Design for Energy Efficiency
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sustainability
Greener Alternative Product
greener alternative category
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
纤维二糖水解酶I可与内切纤维素酶和b-葡萄糖苷酶结合用于从纤维素中产生葡萄糖。
Biochem/physiol Actions
纤维二糖水解酶（CBH）是一种纤维素酶，可通过水解1,4-β-D-糖苷键来降解纤维素。CBH是一种外切纤维素酶，可从纤维素末端切割2至4个单元。CBH I可从还原端逐步切割。CBH I常用于清洁纺织品的洗涤剂中。它在37° C至50° C间存在酶活，最适温度约为45° C。该酶的最适pH为5-6。
General description
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纤维素水解酶 I 是存在于许多真菌（尤其木腐真菌）中的一种酶。它是 53 kDa 的单体，具有催化结构域和纤维素结合结构域。这个反应将水加到纤维素（糖链的非还原端）的葡萄糖键上，生成纤维二糖。
Other Notes
单位定义：在 50° C、pH 为 5 的条件下，1 个单位每分钟将转化 1 nmole 甲基伞形基 β-D 纤维生物糖苷。
Physical form
以硫酸铵沉淀的形式提供，来源为重组玉米。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Jenni Liisa Rahikainen et al.
Bioresource technology, 146, 118-125 (2013-08-08)
Non-productive enzyme adsorption onto lignin inhibits enzymatic hydrolysis of lignocellulosic biomass. Three cellobiohydrolases, Trichoderma reesei Cel7A (TrCel7A) and two engineered fusion enzymes, with distinctive modular structures and temperature stabilities were employed to study the effect of temperature on inhibition arising
Nicolaj Cruys-Bagger et al.
The Journal of biological chemistry, 287(22), 18451-18458 (2012-04-12)
The transient kinetic behavior of enzyme reactions prior to the establishment of steady state is a major source of mechanistic information, yet this approach has not been utilized for cellulases acting on their natural substrate, insoluble cellulose. Here, we elucidate
Zachary K Haviland et al.
The Journal of biological chemistry, 297(3), 101029-101029 (2021-08-03)
Understanding the mechanism by which cellulases from bacteria, fungi, and protozoans catalyze the digestion of lignocellulose is important for developing cost-effective strategies for bioethanol production. Cel7A from the fungus Trichoderma reesei is a model exoglucanase that degrades cellulose strands from
Oren Yaniv et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 7), 819-828 (2012-07-04)
The crystal structure of the family 3b carbohydrate-binding module (CBM3b) of the cellulosomal multimodular hydrolytic enzyme cellobiohydrolase 9A (Cbh9A) from Clostridium thermocellum has been determined. Cbh9A CBM3b crystallized in space group P4(1) with four molecules in the asymmetric unit and
Nicolaj Cruys-Bagger et al.
Biotechnology and bioengineering, 109(12), 3199-3204 (2012-07-07)
An amperometric enzyme biosensor for continuous detection of cellobiose has been implemented as an enzyme assay for cellulases. We show that the initial kinetics for cellobiohydrolase I, Cel7A from Trichoderma reesei, acting on different types of cellulose substrates, semi-crystalline and
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