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重组
expressed in corn
质量水平
形式
liquid
比活
0.13 U/mg
环保替代产品特性
Design for Energy Efficiency
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sustainability
Greener Alternative Product
环保替代产品分类
运输
dry ice
储存温度
−20°C
一般描述
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纤维素水解酶 I 是存在于许多真菌（尤其木腐真菌）中的一种酶。它是 53 kDa 的单体，具有催化结构域和纤维素结合结构域。这个反应将水加到纤维素（糖链的非还原端）的葡萄糖键上，生成纤维二糖。
应用
纤维二糖水解酶I可与内切纤维素酶和b-葡萄糖苷酶结合用于从纤维素中产生葡萄糖。
生化/生理作用
纤维二糖水解酶（CBH）是一种纤维素酶，可通过水解1,4-β-D-糖苷键来降解纤维素。CBH是一种外切纤维素酶，可从纤维素末端切割2至4个单元。CBH I可从还原端逐步切割。CBH I常用于清洁纺织品的洗涤剂中。它在37° C至50° C间存在酶活，最适温度约为45° C。该酶的最适pH为5-6。
单位定义
单位定义：在 50° C、pH 为 5 的条件下，1 个单位每分钟将转化 1 nmole 甲基伞形基 β-D 纤维生物糖苷。
外形
以硫酸铵沉淀的形式提供，来源为重组玉米。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Nicolaj Cruys-Bagger et al.
Biotechnology and bioengineering, 109(12), 3199-3204 (2012-07-07)
An amperometric enzyme biosensor for continuous detection of cellobiose has been implemented as an enzyme assay for cellulases. We show that the initial kinetics for cellobiohydrolase I, Cel7A from Trichoderma reesei, acting on different types of cellulose substrates, semi-crystalline and
Zachary K Haviland et al.
The Journal of biological chemistry, 297(3), 101029-101029 (2021-08-03)
Understanding the mechanism by which cellulases from bacteria, fungi, and protozoans catalyze the digestion of lignocellulose is important for developing cost-effective strategies for bioethanol production. Cel7A from the fungus Trichoderma reesei is a model exoglucanase that degrades cellulose strands from
Oren Yaniv et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 7), 819-828 (2012-07-04)
The crystal structure of the family 3b carbohydrate-binding module (CBM3b) of the cellulosomal multimodular hydrolytic enzyme cellobiohydrolase 9A (Cbh9A) from Clostridium thermocellum has been determined. Cbh9A CBM3b crystallized in space group P4(1) with four molecules in the asymmetric unit and
Larissa C Textor et al.
The FEBS journal, 280(1), 56-69 (2012-11-02)
Aiming to contribute toward the characterization of new, biotechnologically relevant cellulolytic enzymes, we report here the first crystal structure of the catalytic core domain of Cel7A (cellobiohydrolase I) from the filamentous fungus Trichoderma harzianum IOC 3844. Our structural studies and
Svein J Horn et al.
Methods in enzymology, 510, 69-95 (2012-05-23)
Natural cellulolytic enzyme systems as well as leading commercial cellulase cocktails are dominated by enzymes that degrade cellulose chains in a processive manner. Despite the abundance of processivity among natural cellulases, the molecular basis as well as the biotechnological implications
商品
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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