分析物化学类别
proteins (GST)
技术
immunoprecipitation (IP): suitable
protein purification: suitable
储存温度
2-8°C
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一般描述
EZviewä Red谷胱甘肽亲和凝胶是一种易于观察的红色谷胱甘肽琼脂糖亲和凝胶。亲和树脂由通过硫共价连接到环氧活化的4%交联琼脂糖珠上的谷胱甘肽组成，形成12个原子的间隔基。
应用
该产品用于亲和捕获，分子下拉或IP包含谷胱甘肽结合序列的蛋白质，例如谷胱甘肽S-转移酶，谷胱甘肽过氧化物酶和乙二醛酶I。
在小规模亲和捕获中，如免疫沉淀，难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂，会导致结果产生数量差异。EZview™ 红色亲和凝胶可大大降低沉淀损失的风险。 EZview™树脂的性能与传统无色亲和凝胶相同，但更易于区分沉淀和上清液。 这将获得更准确的数据，因为蛋白质损失减少。
特点和优势
- 可见性增加-红色可降低意外吸入的风险
- 通过减少意外损失提高目标蛋白的回收率
- 重现性更高-收率更一致
外形
含有50%甘油和15 ppm Kathon的PBS的1:1（v/v）混悬液。
法律信息
EZview is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
10 - Combustible liquids
WGK
WGK 3
法规信息
动植物源性产品
P L Hallauer et al.
BMC genetics, 1, 1-1 (2000-10-19)
Versatile transgenic manipulation of skeletal muscle requires knowledge of the expression profiles of diverse promoter/enhancer elements in the transcriptionally specialized fiber types of which muscle is composed. "Universal" viral promoters/enhancers, e.g., cytomegalovirus IE1 (CMV IE1), are of interest as reagents
S K Jang et al.
Journal of virology, 62(8), 2636-2643 (1988-08-01)
Picornavirus RNAs are uncapped messengers and have unusually long 5' nontranslated regions (5'NTRs) which contain many noninitiating AUG triplets. The translational efficiency of different picornavirus RNAs varies between different cell-free extracts and even in the same extract, such as micrococcal
S Andersson et al.
The Journal of biological chemistry, 264(14), 8222-8229 (1989-05-15)
The conversion of cholesterol into bile acids in the liver represents the major catabolic pathway for the removal of cholesterol from the body. In this complex biosynthetic pathway, at least 10 enzymes modify both the ring structure and side chain
D R Thomsen et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(3), 659-663 (1984-02-01)
The DNA templates containing immediate early (IE) genes of human cytomegalovirus (CMV) were transcribed in vitro by using a HeLa cell extract. When IE region 1, 2, and 3 were used, transcription was detected qualitatively only from IE region 1.
相关内容
利用亲和力或GST沉降、串联亲和纯化（TAP）和免疫共沉淀法研究体外蛋白-蛋白相互作用所需的pull-down检测方法、试剂和实验方案。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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