推荐产品
生物来源
mouse
质量水平
重组
expressed in E. coli
检测方案
≥90% (SDS-PAGE)
形式
lyophilized powder
效能
0.05-1 ng/mL ED50/EC50
分子量
~6 kDa
包装
pkg of 100 μg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
≤1 EU/μg endotoxin (Protein)
颜色
white
溶解性
water: soluble 0.10 mL, clear, colorless
UniProt登记号
储存温度
−20°C
基因信息
mouse ... Egf(13645)
相关类别
一般描述
表皮生长因子（EGF）是小的促有丝分裂多肽（∼6 kDa），存在于许多哺乳动物物种中，分布在许多组织和体液中。四种ErbB (HER) 家族受体酪氨酸激酶(包括EGFR/ErbB1、ErbB2、 ErbB3和 ErbB4）介导针对EGF家族成员的反应。人类和小鼠的EGF非常相似，但是它们的物理和化学性质并不相同。在构成两种多肽中每一种多肽的53个氨基酸残基中，有37个是两种分子共有的，并且在相同的相对位置上形成三个二硫键。
应用
来自小鼠的表皮生长因子已用作基础（N2B27）培养基补充剂，用于培养分化的小鼠胚胎干（E14）细胞。
生化/生理作用
EGF（表皮生长因子）可参与细胞增殖、分化和存活。 此外，它还可影响各种生物活动，如血管生成、抑制胃酸分泌、调节多种激素的合成、细胞外基质蛋白质的合成和转换、骨组织钙释放（从而促进骨吸收）、成纤维细胞和上皮细胞的化学趋化作用、单独或与其他细胞因子组合介导伤口愈合过程。 EGF对于多种细胞类型具有促有丝分裂作用，包括成纤维细胞、上皮细胞、内皮细胞、软骨细胞、以及SV40-3T3细胞。
外形
该产品从磷酸盐缓冲盐水（pH 7.4，0.2 μm滤膜过滤）中冻干。
引用
1. Carpenter, G., and Cohen, S., Epidermal growth factor. Annu. Rev. Biochem., 48, 193-216 (1979).
2. Jorissen, R.N., et al., Epidermal growth factor: mechanisms of activation and signaling. Exp. Cell Res., 284, 31-53 (2003).
3. Herbst, R.S., Review of epidermal growth factor receptor biology. Int.J. Radiat. Oncol. Biol., Phys., 59, S21-S26 (2004).
4. Mehta, V.B., and Besner, G.E., HB-EGF promotes angiogenesis in endothelial cells via PI3-kinase and MAPK signaling pathways. Growth Factors, 25, 253-263 (2007).
5. Bower, J.M., et al., The inhibition of gastric acid secretion by epidermal growth factor. Experientia, 31, 825-826 (1975).
6. Schonbrunn, A., et.al., Epidermal growth factor and thyrotropin-releasing hormone act similarly on a clonal pituitary cell strain. Modulation of hormone production and inhibition of cell proliferation. J. Cell Biol., 85, 786-797 (1980).
7. Mimura, Y., et al., Epidermal growth factor induces fibronectin expression in human dermal fibroblasts via protein kinase C delta signaling pathway. J. Invest. Dermatol., 122, 1390-1398 (2004).
8. Warner, M.R. et al., Ametantrone inhibits prostaglandin-mediated resorption in bone organ culture. Prostaglandins, 28, 469-476 (1984).
9. Grotendorst, G.R., et al., EGF and TGF-alpha are potent chemoattractants for endothelial cells and EGF-like peptides are present at sites of tissue regeneration. J. Cell. Physiol., 139, 617623 (1989).
10. Schultz, G., et al., EGF and TGF-alpha in wound healing and repair. J. Cell. Biochem., 45, 346-352 (1991).
11. George-Nascimento, C., et al., Characterization of recombinant human epidermal growth factor produced in yeast. Biochemistry, 27, 797-802 (1988).
2. Jorissen, R.N., et al., Epidermal growth factor: mechanisms of activation and signaling. Exp. Cell Res., 284, 31-53 (2003).
3. Herbst, R.S., Review of epidermal growth factor receptor biology. Int.J. Radiat. Oncol. Biol., Phys., 59, S21-S26 (2004).
4. Mehta, V.B., and Besner, G.E., HB-EGF promotes angiogenesis in endothelial cells via PI3-kinase and MAPK signaling pathways. Growth Factors, 25, 253-263 (2007).
5. Bower, J.M., et al., The inhibition of gastric acid secretion by epidermal growth factor. Experientia, 31, 825-826 (1975).
6. Schonbrunn, A., et.al., Epidermal growth factor and thyrotropin-releasing hormone act similarly on a clonal pituitary cell strain. Modulation of hormone production and inhibition of cell proliferation. J. Cell Biol., 85, 786-797 (1980).
7. Mimura, Y., et al., Epidermal growth factor induces fibronectin expression in human dermal fibroblasts via protein kinase C delta signaling pathway. J. Invest. Dermatol., 122, 1390-1398 (2004).
8. Warner, M.R. et al., Ametantrone inhibits prostaglandin-mediated resorption in bone organ culture. Prostaglandins, 28, 469-476 (1984).
9. Grotendorst, G.R., et al., EGF and TGF-alpha are potent chemoattractants for endothelial cells and EGF-like peptides are present at sites of tissue regeneration. J. Cell. Physiol., 139, 617623 (1989).
10. Schultz, G., et al., EGF and TGF-alpha in wound healing and repair. J. Cell. Biochem., 45, 346-352 (1991).
11. George-Nascimento, C., et al., Characterization of recombinant human epidermal growth factor produced in yeast. Biochemistry, 27, 797-802 (1988).
WGK
WGK 1
法规信息
常规特殊物品
Epidermal growth factor: mechanisms of activation and signaling.
Jorissen RN et al.
Experimental Cell Research, 284, 31-31 (2003)
Characterization of recombinant human epidermal growth factor produced in yeast.
George-Nascimento C et al.
Biochemistry, 27, 797-797 (1988)
EGF and TGF-alpha are potent chemoattractants for endothelial cells and EGF-like peptides are present at sites of tissue regeneration.
Grotendorst GR et al.
Journal of Cellular Physiology, 139, 617623 -617623 (1989)
The inhibition of gastric acid secretion by epidermal growth factor.
Bower JM et al.
Experientia, 31, 825-825 (1975)
Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells
Semrau S, et al.
Nature Communications, 8(1), 1096-1096 (2017)
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