推荐产品
生物来源
mouse
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
ELKS-30, monoclonal
形式
buffered aqueous solution
分子量
antigen ~120 kDa
种属反应性
rat, chicken, bovine, human, mouse
浓度
~2 mg/mL
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa cell extract
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ERC1(23085)
mouse ... Erc1(111173)
一般描述
ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap.
Monoclonal Anti-ELKS (mouse IgG2a isotype) is derived from the hybridoma ELKS-30 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human ELKS. ELKS, also known as KIAA1081, is an essential regulatory subunit of the IKK complex. It was named ELKS since 44.1% of the sequence is composed of glutamic acid (E), leucine (L), lysine (K) and serine (S) residues. The protein contains 948 amino acids with nine -helical coiled-coil domains including periodic heptad repeats that predict dimer formation. Its highest expression is in heart, placenta, lung, brain, thyroid and testis.
应用
Monoclonal Anti-ELKS antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- fluorescence immunohistochemistry
- immunocytochemistry
- immunoblotting
生化/生理作用
ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap. ERC1 encodes a regulator of neurotransmitter release and acts as the best gene candidate contributing to this phenotype as well as to the autism spectrum disorder ASD. ERC1a (an isoform of ERC1) is associated with other proteins for effective migration and tumor cell invasion by stabilizing the activity at the cell front. Depletion of any of the associated protein negatively affects invasion, migration on extracellular matrix, lamellipodial persistence and the internalization of active integrin β1 receptors needed for adhesion turnover at the front of the cell.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Fusion of a novel gene, ELKS, to RET due to translocation t (10; 12)(q11; p13) in a papillary thyroid carcinoma
Genes Chromosomes Cancer, 25(2), 97-103 (1999)
12p13.33 microdeletion including ELKS/ERC1, a new locus associated with childhood apraxia of speech.
European journal of human genetics : EJHG, 21(1), 82-88 (2012-06-21)
Speech sound disorders are heterogeneous conditions, and sporadic and familial cases have been described. However, monogenic inheritance explains only a small proportion of such disorders, in particular in cases with childhood apraxia of speech (CAS). Deletions of <5 Mb involving
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(2), 512-525 (2011-01-14)
Synapse formation requires the organization of presynaptic active zones, the synaptic vesicle release sites, in precise apposition to postsynaptic neurotransmitter receptor clusters; however, the molecular mechanisms responsible for these processes remain unclear. Here, we show that P/Q-type and N-type voltage-dependent
The Journal of biological chemistry, 290(21), 13372-13385 (2015-04-12)
DNA damage-induced NF-κB activation plays a critical role in regulating cellular response to genotoxic stress. However, the molecular mechanisms controlling the magnitude and duration of this genotoxic NF-κB signaling cascade are poorly understood. We recently demonstrated that genotoxic NF-κB activation
eLife, 11 (2022-05-14)
A developing understanding suggests that spatial compartmentalisation in pancreatic β cells is critical in controlling insulin secretion. To investigate the mechanisms, we have developed live-cell subcellular imaging methods using the mouse organotypic pancreatic slice. We demonstrate that the organotypic pancreatic
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