E4156
抗 早期内涵体抗原 1（N-末端） 兔抗
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-EEA1, Anti-Endosome-associated Protein p162, Anti-Zinc Finger FYVE Domain-containing Protein 2
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB CL
Citations:
15
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~160 kDa
species reactivity
mouse, human, rat
concentration
~1 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using human HeLa and rat NRK cells, western blot (chemiluminescent): 0.4-0.8 μg/mL using whole extract of mouse NIH-3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... EEA1(8411)
mouse ... Eea1(216238)
rat ... Eea1(314764)
General description
The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic face of early endosomes. It is a 162 kDa autoantigen protein. EEA1 is a dimer, which comprises extensive coiled-coil regions. At its C-terminus, it contains a cysteine-rich zinc-finger-like domain named FYVE domain. This FYVE domain is conserved from yeast to man. FYVE domain is implicated in the specific localization of EEA1 to endosomes.
Immunogen
synthetic peptide corresponding amino acid residues 24-40 of human EEA1 with C-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.
Application
Anti-Early Endosomal Antigen 1 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Early Endosomal Antigen 1 (EEA1) localization in endosomes is implicated in subacute systemic lupus erythematosus. Endosomal targeting of EEA1 also requires its binding to the active form of the small GTPase Rab5. The binding of EEA1 to phosphatidylinositol 3 phosphate (PtdInsP) and rabaptin-5 (Rab5)-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. Anti-EEA1 may be used as an early endosome marker.
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
Stefanie Hetzenecker et al.
Traffic (Copenhagen, Denmark), 17(4), 351-368 (2015-12-10)
Human cytomegalovirus (HCMV) is an important and widespread pathogen in the human population. While infection by this β-herpesvirus in endothelial, epithelial and dendritic cells depends on endocytosis, its entry into fibroblasts is thought to occur by direct fusion of the
Lipid interaction networks of peripheral membrane proteins revealed by data-driven micelle docking
Dancea F, et al.
Biophysical Journal, 94(2), 515-524 (2008)
Distinct lysosomal network protein profiles in Parkinsonian syndrome cerebrospinal fluid
Boman A, et al.
Journal of Parkinson's Disease, 6(2), 307-315 (2016)
Andrea Boman et al.
Journal of Parkinson's disease, 6(2), 307-315 (2016-04-12)
Clinical diagnosis of parkinsonian syndromes like Parkinson's disease (PD), corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP) is hampered by overlapping symptomatology and lack of diagnostic biomarkers, and definitive diagnosis is only possible post-mortem. Since impaired protein degradation plays an
FYVE and coiled-coil domains determine the specific localisation of Hrs to early endosomes
Raiborg C, et al.
Journal of Cell Science, 114(12), 2255-2263 (2001)
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