推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 35 kDa
种属反应性
rat, mouse, bovine, pig, canine, human
浓度
~1.5 mg/mL
技术
immunoprecipitation (IP): 10-20 μg using cell lysate of the human epitheloid carcinoma HeLa cell line
indirect immunofluorescence: 1-2 μg/mL using rat fibroblast Rat1 cells
indirect immunofluorescence: suitable
western blot: 0.5-1 μg/mL using cell lysate of the rat fibroblast cell line Rat1 or Rat 2
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MAPRE1(22919)
mouse ... Mapre1(13589)
rat ... Mapre1(114764)
一般描述
Microtubule-associated protein RP/EB family member 1 is a protein encoded by the MAPRE1 gene in humans. EB1 (End-Binding-1) proteins belong to a distinct family of accessory proteins, termed “plus-end tracking proteins” or +TIPs, that bind to the plus-ends of microtubules. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.
免疫原
synthetic peptide corresponding to amino acids 252-268 located at the C-terminus of human EB1, conjugated to KLH. This sequence is identical in several species (mouse, rat, dog, bovine, and porcine EB1), highly conserved (single amino acid substitution) in chicken EB1, and has limited homology to human EB2 (35% identity) and human EB3 (53% identity).
应用
Anti-EB1 antibody produced in rabbit has been used in
- immunofluorescence
- western blotting
- immunocytochemistry
- immunoprecipitation
生化/生理作用
End-Binding-1 (EB1) associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.
The C-terminal domain of End-binding proteins (EBs) is important for its autonomous microtubule tip tracking, regulation of microtubule dynamics, chromosome segregation and recruitment of numerous partners to microtubule ends. EB1 proteins bind to microtubule ends and act with the adenomatous polyposis coli (APC) tumor suppressor to regulate the microtubule filament system. It is a potential oncogene and plays an important role during early event in colon carcinogenesis, colorectal cancer (CRC). EB1 acts as a potential biomarker and therapeutic target in CRC.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
EB1 contributes to proper front-to-back polarity in neutrophil-like HL-60 cells
Samereier M, et al.
European Journal of Cell Biology, 96(2), 143-153 (2017)
EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability
Tirnauer JS and Bierer BE
The Journal of cell biology, 149(4), 761-766 (2000)
Cell-length-dependent microtubule accumulation during polarization
Seetapun D and Odde DJ
Current Biology, 20(11), 979-988 (2010)
Dominique Seetapun et al.
Current biology : CB, 20(11), 979-988 (2010-05-25)
Breaking cell symmetry, known as polarization, requires dynamic reorganization of microtubules (MTs) and is essential to many cellular processes, including axon formation in neurons. A critical step in polarization is believed to be the "selective stabilization" of MTs, which hypothesizes
The Leber congenital amaurosis protein AIPL1 and EB proteins co-localize at the photoreceptor cilium
Hidalgo-de-Quintana J, et al.
PLoS ONE, 10(3), e0121440-e0121440 (2015)
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