形式
liquid
质量水平
分析物化学类别
proteins (Immunoglobulins of various mammalian species)
技术
affinity chromatography: suitable
immunoprecipitation (IP): suitable
容量
≥8 mg/mL, gel binding capacity (rabbit IgG)
储存温度
−20°C
一般描述
EZ view Red蛋白G亲和凝胶通过共价键与4%琼脂糖珠结合。该亲和凝胶用于将抗体IgG的FC部分与样品结合，如细胞裂解物和组织，纯化标记蛋白以进行免疫沉淀分析。红色染料增强了可视性，以获得更有效的结果。
应用
在小规模亲和捕获中，如免疫沉淀，难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂，会导致结果产生数量差异。EZview™ 红色亲和凝胶可大大降低沉淀损失的风险。 EZview树脂的性能与传统无色亲和凝胶相同，但更易于区分沉淀和上清液。 这将获得更准确的数据，因为蛋白质损失减少。
特点和优势
- 可见性增加-红色可降低意外吸入的风险
- 通过减少意外损失提高目标蛋白的回收率
- 重现性更高-收率更一致
外形
1:1（v/v）混悬液溶于含有50%甘油和15 ppm Kathon的PBS中。
PBS中50%悬浮液加入50%甘油及0.0015%
法律信息
EZview is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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Nature communications, 8(1), 2029-2029 (2017-12-13)
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Epigenetic mechanisms, including chromatin structure, chromatin dynamics and histone modifications play an important role for maintenance and differentiation of pluripotent embryonic stem cells. However, little is known about the molecular mechanisms of adult stem cell specification and differentiation. Here, we
Min Mo et al.
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The anaphase-promoting complex (APC), or cyclosome, is a ubiquitin ligase with major roles in cell cycle regulation. It is required for mitotic exit, but must be deactivated for the G(1)/S phase transition to occur. APC consists of at least 12
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The Journal of biological chemistry, 295(33), 11764-11775 (2020-06-27)
Macrophages play critical roles in homeostasis and inflammation. Macrophage polarization to either a pro-inflammatory or anti-inflammatory status is controlled by activating inflammatory signaling pathways. Ubiquitination is a posttranslational modification that regulates these inflammatory signaling pathways. However, the influence of protein
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