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质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
phenol red: no
NaHCO3: 2.2 g/L
glucose: 1.0 g/L (Dextro)
运输
ambient
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一般描述
Earle′s平衡盐溶液(EBSS)是生理pH范围内的盐溶液,可促进细胞自噬。通过上调分化簇(CD86)、人白细胞抗原-DR亚型(HLA-DR)和白介素(IL-6),增强卡介苗(BCG)诱导的树突状细胞(DC)成熟。
应用
Earle′s平衡盐溶液已用于培养单层Caco-2细胞以诱导饥饿反应,可搭配HEPES缓冲液检测胰蛋白酶处理细胞中的NADPH氧化酶活性。也用于处理CEM RES细胞,进行氨基酸缺乏实验。
其他说明
酚红已经被证明在克隆密度下会干扰一些细胞的生长。当处理干细胞或以低密度培养细胞时,可使用这种培养基。
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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