conjugate
TRITC conjugate
form
buffered aqueous solution
extent of labeling
1-5 mol fluorochrome per mol protein
technique(s)
immunohistochemistry: 100 μg/mL using human appendix/tonsil sections and Monoclonal Anti Actin, α-Smooth Muscle as primary antibody, indirect immunofluorescence: 1:200
shipped in
dry ice
storage temp.
−20°C
Application
ExtrAvidin®-TRITC is suitable for indirect immunofluorescence at a dilution of 1:200.
Biochem/physiol Actions
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and ow background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin® has been conjugated to tetramethylrhodamine isothiocyanate (TRITC) and is used for various techniques.
Physical form
Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5 and 15 mM sodium azide
Preparation Note
Affinity purified protein
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Cdc42 and the Ste20-like kinase Don3 act independently in triggering cytokinesis in Ustilago maydis.
Christian Böhmer et al.
Journal of cell science, 121(Pt 2), 143-148 (2007-12-20)
In the dimorphic fungus Ustilago maydis the Rho-family GTP-binding protein Cdc42 and the Ste20-like kinase Don3 are both essential for triggering cell separation during cytokinesis. Since Don3 does not contain a Cdc42/Rac interaction and binding domain (CRIB), it is unclear
Niki Kourmouli et al.
Journal of cell science, 117(Pt 12), 2491-2501 (2004-05-07)
Tri-methylated lysine 20 on histone H4 (Me(3)K20H4) is a marker of constitutive heterochromatin in murine interphase and metaphase cells. Heterochromatin marked by Me(3)K20H4 replicates late during S phase of the cell cycle. Serum starvation increases the number of cells that
Yusuke Sakai et al.
Journal of tissue engineering and regenerative medicine, 12(12), 2287-2298 (2018-10-26)
Subcutaneous transplantation of engineered hepatocyte/fibroblast sheets (EHFSs) is a low invasive and safe approach to construct vascularized subcutaneous human liver tissue (VSLT). However, the liver-specific structures and functionalities in the development process of VSLTs in mice remain poorly understood. Here
H Daryabari et al.
Poultry science, 93(9), 2289-2295 (2014-07-09)
Published data on the probable involvement of avidin and avidin-related protein-2 (AVR2) in sustaining sperm viability in sperm storage tubules in 38-wk-old turkeys, and the high affinity of avidin or its analogs to biotin suggest that supplementary biotin may increase
Melanie Köhler et al.
Molecules (Basel, Switzerland), 19(8), 12531-12546 (2014-08-26)
Avidin and avidin-like proteins are widely used in numerous techniques since the avidin-biotin interaction is known to be very robust and reliable. Within this study, we investigated this bond at the molecular level under harsh conditions ranging from very low
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