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偶联物
FITC conjugate
质量水平
形式
buffered aqueous solution
标记范围
3-6 mol fluorochrome per mol protein
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues
indirect immunofluorescence: 1:200
运输
dry ice
储存温度
−20°C
一般描述
亲和素是一种四聚或二聚生物素结合蛋白，在鸟类、爬行动物和两栖动物的输卵管中产生，并沉积在卵白中。它由四个生物素的高亲和力结合位点组成。外膜蛋白由蛋清亲和素制备而成。它是亲和纯化亲和素的改性形式，结合亲和素的高比活性和链霉亲和素的低背景染色。它是一种生物素结合蛋白产生的细菌Streptomyces avidinii。ExtrAvidin已与荧光素异硫氰酸酯(FITC)结合，并用于各种技术。
应用
ExtrAvidin®-FITC适用于以下应用：
- 免疫组化（福尔马林固定，石蜡包埋切片），浓度为20μg/mL，使用人体组织。
- 免疫荧光†
- 竞争结合试验
外形
0.01 M磷酸盐缓冲盐水溶液，包含10% (v/v) 0.5 M碳酸盐缓冲液和15 mM叠氮化钠溶液。
制备说明
亲和纯化蛋白
法律信息
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Maria Cristina D Picardo et al.
The Journal of physiology, 591(10), 2687-2703 (2013-03-06)
Breathing in mammals depends on an inspiratory-related rhythm that is generated by glutamatergic neurons in the pre-Bötzinger complex (preBötC) of the lower brainstem. A substantial subset of putative rhythm-generating preBötC neurons derive from a single genetic line that expresses the
D R Hampson et al.
The Journal of biological chemistry, 274(47), 33488-33495 (1999-11-24)
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Xueying Wang et al.
eLife, 3, e03427-e03427 (2014-07-17)
To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm
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