E2759
内皮细胞生长补充剂 来源于牛神经组织
powder, suitable for cell culture
别名:
ECGS, Endothelial Mitogen
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关于此项目
UNSPSC Code:
12352202
NACRES:
NA.77
Biological source:
bovine neuroblastoma
Form:
powder
Impurities:
≤20 EU/vial (LAL test)
产品名称
内皮细胞生长补充剂 来源于牛神经组织, ECGS, suitable for cell culture
biological source
bovine neuroblastoma
form
powder
potency
2-200 μg/mL ED50/EC50
quality
endotoxin tested
packaging
pkg of 15 and 5X15 mg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤20 EU/vial (LAL test)
color
tan to brown
solubility
water: soluble (1 vial reconstituted with 1 ml 0.85% NaCl)
storage temp.
2-8°C
Quality Level
Analysis Note
内皮细胞生长补充剂（ECGS）的增殖活性是通过使用低密度接种的胎牛心脏内皮细胞（ATCC CRL 1395）而在培养中进行测试的。EC50被定义为在基于细胞的生物测定中可引起细胞生长增加50％的生长因子有效浓度。
Application
来自牛神经组织的内皮细胞生长补充剂已作为杜氏′改良Eagles′培养基补充剂和F-12K培养基补充剂，用于人脐静脉内皮细胞培养。
Biochem/physiol Actions
内皮细胞生长补充剂（ECGS）可改善人隐静脉内皮细胞（HSVEC）和人脐静脉内皮细胞（HUVEC）的增殖。它可与白介素1（IL-1）协调并介导血管平滑肌细胞（VSMC）中的基质重塑。它对于内皮细胞增殖和脂肪干细胞（ASC）的分化至关重要。包含哺乳动物血管内皮细胞生长促进因子的牛神经组织提取物。据报道，ECGS可作为培养基补充剂在单克隆抗体生产的杂交瘤细胞融合和生长中发挥积极作用。
General description
内皮细胞生长补充剂（ECGS）包含酸性成纤维细胞生长因子（aFGF）和碱性FGF（bFGF）。
Preparation Note
ECGS是一种包含哺乳动物血管内皮细胞生长促进因子的牛神经组织提取物。内皮细胞生长补充剂是通过对Maciag等人的方法进行修改而制备的，并从含有NaCl和硫酸链霉素的无菌溶液进行冻干的。
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
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Heparin/endothelial cell growth supplement regulates matrix gene expression and prolongs life span of vascular smooth muscle cells through modulation of interleukin-1
Hsu JY, et al.
In Vitro Cellular & Developmental Biology. Animal, 35(10), 647-654 (1999)
Endothelial differentiation of adipose-derived stem cells: effects of endothelial cell growth supplement and shear force
Fischer LJ, et al.
The Journal of Surgical Research, 152(1), 157-166 (2009)
T Maciag et al.
The Journal of biological chemistry, 257(10), 5333-5336 (1982-05-25)
Endothelial cell growth factor (ECGF) is a neural-derived mitogen capable of stimulating the proliferation of quiescent populations of human umbilical vein endothelial cells (HUV-EC). ECGF has been isolated from bovine hypothalamus in two forms: a high molecular weight from (high
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