生物来源
mouse
质量水平
重组
expressed in NSO cells
无菌性
sterile; sterile-filtered
检测方案
>90% (SDS-PAGE)
形式
lyophilized powder
分子量
monomer calculated mol wt 88.2 kDa
~120 kDa by SDS-PAGE (reducing)
calculated mol wt 88.2 kDa
包装
pkg of 50 μg
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
UniProt登记号
运输
ambient
储存温度
−20°C
基因信息
mouse ... Cdh1(12550)
一般描述
E (epithelial)-cadherin is a type I classical cadherin, which exists as a dimer. It has an ectodomain made of five extracellular cadherin-like (EC) domains, connected by conserved linker regions, which are calcium-binding in nature. It spans the membrane once, and contains a cytoplasmic region. It is a glycoprotein, and in humans has a molecular weight of 120kDa. It binds to catenin and cytoskeletal protein with its C-terminal domain.
Extracellular domain of mouse E-Cadherin (amino acids 1-709) fused to the carboxy-terminal 6X histidine tagged Fc region of human IgG1.
应用
E-Cadherin/Fc Chimera from mouse has been used in adhesion assays.
生化/生理作用
E (epithelial)-cadherin is involved in hemophilic cell-cell adhesion in a calcium-dependent manner. It forms adheren junctions through its ectodomains, to maintain physical contacts between adjacent cells. It plays essential roles in developmental processes, tissue homeostasis and multiple diseases. In humans, this protein might be involved in the pathophysiology of ulcerative colitis. In Crohn′s diseased patients, mutations in this gene lead to a truncated or mislocalized protein product. Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) are the underlying processes of tumorigenesis and embryogenesis, and these involve the control of E-cadherin expression.
外形
Lyophilized from 0.2 μm filtered solution in 20mM MES, 300mM NaCl, 1mM CaCl2.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Xenopus δ-catenin is essential in early embryogenesis and is functionally linked to cadherins and small GTPases.
Gu D, et al.
Journal of Cell Science, 122(22), 4049-4061 (2009)
Dongmin Gu et al.
Journal of cell science, 122(Pt 22), 4049-4061 (2009-10-22)
Catenins of the p120 subclass display an array of intracellular localizations and functions. Although the genetic knockout of mouse delta-catenin results in mild cognitive dysfunction, we found severe effects of its depletion in Xenopus. delta-catenin in Xenopus is transcribed as
Ying Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(41), 16462-16467 (2013-09-27)
Epithelial cadherin (E-cadherin), a member of the classical cadherin family, mediates calcium-dependent homophilic cell-cell adhesion. Crystal structures of classical cadherins reveal an adhesive dimer interface featuring reciprocal exchange of N-terminal β-strands between two protomers. Previous work has identified a putative
Hani Alotaibi et al.
Biochimica et biophysica acta, 1849(6), 731-742 (2015-02-06)
Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) highlight crucial steps during embryogenesis and tumorigenesis. Induction of dramatic changes in gene expression and cell features is reflected by modulation of Cdh1 (E-cadherin) expression. We show that Cdh1 activity during MET is
M J Bussemakers et al.
Molecular biology reports, 17(2), 123-128 (1993-02-01)
E-cadherin is a Ca(2+)-dependent cell adhesion molecule involved in cell-cell interaction. In its normal physiological function it plays an important role in embryonic development and tissue morphogenesis. Recent studies have shown that in cancer development E-cadherin can act as a
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