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应用
已经过高度纯化以去除干扰性β-葡糖醛酸糖苷酶和蛋白酶活性，可用于硫酸软骨素水解和 HPLC 分析。
来自肝素黄杆菌的软骨素酶AC是一种通过消除机制将己糖胺和葡萄糖醛酸残基之间具有（1-4）键的硫酸化和非硫酸化多糖链裂解的酶。 所得到的寡糖产物主要是具有不饱和糖醛酸的二糖。 软骨素酶AC可特异性地降解硫酸软骨素A和C，但不可降解硫酸软骨素B（硫酸皮肤素）。
软骨素酶AC已用于商业样品（例如膳食补充剂）中硫酸软骨素的分析。
单位定义
在37℃，pH 6.7条件下，1单位是指每分钟从硫酸软骨素A中释放1.0μmole不饱和双糖所需的酶量（通过A232的变化进行测定）。εμΜ值对于反应产物Δ-Di-4S（硫酸软骨素A和B）来说为5.1，对于Δ-Di-6S（硫酸软骨素C）来说为5.5。
其他说明
查看有关 用于复合碳水化合物分析的酶 的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Determination of Chondroitin Sulfate Content in Raw Materials and Dietary Supplements by High-Performance Liquid Chromatography with Ultraviolet Detection After Enzymatic Hydrolysis: Single-Laboratory Validation
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Journal - Association of Official Analytical Chemists, 90(3), 659-669 (2007)
E M Denholm et al.
European journal of pharmacology, 416(3), 213-221 (2001-04-06)
In the current study, two specific glycosaminoglycan lyases, chondroitinase AC and chondroitinase B, were utilized to examine the roles of chondroitin sulfates and dermatan sulfate in tumor metastasis and angiogenesis. Melanoma cells (SK-MEL) or endothelial cells were treated with either
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K Pojasek et al.
Biochemical and biophysical research communications, 286(2), 343-351 (2001-08-14)
Glycosaminoglycans (GAGs) are a family of complex polysaccharides involved in a diversity of biological processes, ranging from cell signaling to blood coagulation. Chondroitin sulfate (CS) and dermatan sulfate (DS) comprise a biologically important subset of GAGs. Two of the important
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Enterococcus faecium is a multidrug-resistant (MDR) nosocomial pathogen causing significant morbidity in debilitated patients. New antimicrobials are needed to treat antibiotic-resistant E. faecium infections in hospitalised patients. E. faecium incorporates lipoteichoic acid (LTA) (1,3-polyglycerol-phosphate linked to glycolipid) in its cell
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Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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