推荐产品
产品名称
内皮细胞生长补充剂 来源于牛脑垂体, ECGS, suitable for cell culture, BioReagent
生物来源
bovine pituitary glands
质量水平
产品线
BioReagent
表单
powder
效能
2-300 μg/mL ED50/EC50
质量
endotoxin tested
包装
pkg of 15 mg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
≤20 EU/vial (LAL test)
颜色
brown to tan
溶解性
water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)
储存温度
2-8°C
一般描述
内皮细胞生长补充剂（ECGS）是一种包含哺乳动物血管内皮细胞生长促进因子的牛垂体腺的提取物。内皮细胞生长补充剂使用一种已发表方法的改良方案制备而成。它通过从含有NaCl和硫酸链霉素的无菌溶液冻干而成。
应用
牛垂体内皮细胞生长补充剂已用作：
- 罗斯威尔公园纪念研究所（RPMI）1640培养中中用于内皮细胞培养的组成成分
- 内皮细胞培养培养基199的组成成分
- 杜氏′改良Eagle培养基（DMEM）：Ham′s F-12的补充剂
- Iscove改良DMEM培养基（IMDM）用于人脐静脉内皮细胞（HUVEC）培养的补充剂′′
生化/生理作用
据报道，内皮细胞生长补充剂（ECGS）可作为培养基补充剂在单克隆抗体生产的杂交瘤细胞融合和生长中发挥积极作用。
警示用语：
Warning
危险声明
危险分类
Repr. 2
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
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James F Dylewski et al.
MethodsX, 7, 101048-101048 (2020-09-19)
Glomerular endothelial cells (GEnC) are a specialized microvascular subset of endothelial cells that, when injured, result in many types of diseases within the kidney. Thus, techniques to study GEnC in a cell culture system are important to investigate mechanisms of
Autoantibodies against heat shock protein 60 mediate endothelial cytotoxicity.
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In vitro biosynthesis of leukemia inhibitory factor/human interleukin for DA cells by human endothelial cells: differential regulation by interleukin-1 alpha and glucocorticoids
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Blood, 86(10), 3763-3770 (1995)
Surface staining and cytotoxic activity of heat-shock protein 60 antibody in stressed aortic endothelial cells.
Xu Q, et al.
Circulation Research, 75(6), 1078-1085 (1994)
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