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形式
liquid
质量
2 μmol of each dNTP
特点
hotstart
浓度
50 mM (each dNTP)
颜色
colorless
运输
dry ice
储存温度
−20°C
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一般描述
CleanAmp dNTPs 是 TriLink BioTechnologies 公司的一款产品。CleanAmp dNTPs 是修饰的核苷三磷酸，可阻断 DNA 聚合酶核苷酸掺入。CleanAmp dNTPs 通过典型热启动 PCR 循环条件中的初始加热步骤和随后的变性步骤激活。该过程限制了每个 PCR 循环期间激活的 dNTP 的量，从而更特异和有效地扩增目标产物，并减少甚至消除错配或引物二聚体。CleanAmp dNTP 为各种基于 PCR 的应用提供了比热启动酶更经济的解决方案。
应用
- For PCR amplifications that require reduced non-specific amplification
- For multiplex PCR
- For reduction of primer dimers
- Substitute for dNTPs in any PCR reaction
包装
2 μmoles: 4 vials:
40 μL dATP at 50 mM in buffered glycine solution
40 μL dCTP at 50 mM in buffered glycine solution
40 μL dTTP at 50 mM in buffered glycine solution
40 μL dGTP at 50 mM in buffered glycine solution
40 μL dATP at 50 mM in buffered glycine solution
40 μL dCTP at 50 mM in buffered glycine solution
40 μL dTTP at 50 mM in buffered glycine solution
40 μL dGTP at 50 mM in buffered glycine solution
法律信息
CleanAmp™ dNTPs are provided to buyer with a non-transferrable right from TriLink BioTechnologies, Inc. to use the purchased CleanAmp™ dNTPs in internal research conducted by the buyer, whether the buyer is an academic, non-profit, or for-profit entity. CleanAmp™ dNTPs are not to be used for human therapeutic or commercial clinical diagnostic use. A license is required for any commercial use of CleanAmp™ dNTPs, regardless of the academic or non-profit status of the using entity. Information about commercial licenses for CleanAmp™ dNTPs may be obtained from TriLink BioTechnologies, Inc. Buyer may not use the CleanAmp™ dNTPs to support the filing of a patent application in any country in the world that contains claims directed to CleanAmp™ dNTPs or uses thereof without the express approval of TriLink BioTechnologies, Inc. Buyer′s right to have and use the CleanAmp™ dNTPs will terminate immediately if you fail to comply with these terms and conditions of this agreement.
CleanAmp is a trademark of TriLink BioTechnologies, Inc.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Xinglong Xiao et al.
Journal of virological methods, 187(2), 357-361 (2012-11-13)
Nucleic acid testing (NAT) is valuable for screening blood donors for occult hepatitis B virus (HBV) infection and infection during the window period in countries where HBV is endemic, such as China. An "in-house" NAT (Triplex NAT) was developed for
Franck Court et al.
Nucleic acids research, 39(14), 5893-5906 (2011-04-12)
Parental genomic imprinting at the Igf2/H19 locus is controlled by a methylation-sensitive CTCF insulator that prevents the access of downstream enhancers to the Igf2 gene on the maternal chromosome. However, on the paternal chromosome, it remains unclear whether long-range interactions
Inna Koukhareva et al.
Nucleic acids symposium series (2004), (52)(52), 259-260 (2008-09-09)
Several 3'-ether and 3'-ester derivatives of 2'-deoxyribonucleoside 5'-triphosphates (dNTPs) were prepared. These dNTP derivatives were not substrates for DNA polymerase and did not support primer extension at room temperature. However, by short pre-heating to 95 degrees C in PCR buffer
Natasha Paul et al.
Methods in molecular biology (Clifton, N.J.), 630, 301-318 (2010-03-20)
Hot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been developed that target
Elena Hidalgo Ashrafi et al.
Current protocols in molecular biology, Chapter 15, Unit 15-Unit 15 (2009-10-10)
Hot-start PCR is a technique that improves PCR performance by reducing nonspecific amplification during the initial setup stages of the PCR. This unit describes hot-start PCR protocols which utilize primers containing temperature-sensitive modifications. The introduction of 4-oxo-tetradecyl (OXT) phosphotriester groups
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