跳转至内容
Merck
CN
所有图片(1)

文件

安全信息

DNAQF

Sigma-Aldrich

DNA 定量试剂盒（荧光法）

Quantitation of DNA using bisBenzimide

别名:

dsDNA quantification

登录查看公司和协议定价
所有图片(1)

About This Item

UNSPSC代码:
41105500
NACRES:
NA.84

质量水平

200

用途

sufficient for 750 assays (2 ml each)

技术

nucleic acid detection: suitable

储存温度

−20°C

一般描述

该试剂盒使用荧光染料bisBenzimide（Hoechst 33258）对DNA进行定量所需的所有试剂。该试剂盒可以高灵敏、快速、准确地测定10ng/ml至5μg/ml的双链DNA，即使存在RNA和蛋白质的情况下也是如此。荧光测定法是一种灵敏度高、简单易行的DNA定量方法。荧光染料bisBenzimide（Hoechst 33258）主要结合双链DNA（dsDNA）小沟内的AT序列，专门用于量化DNA的毫微克量（10 ng/ml至10 mg/ml）。当在360 nm激发时，DNA环境下染料在460 nm处的荧光发射显著增加。

应用

DNA定量试剂盒，荧光法已用于测量DNA浓度/含量：
  • 新生大鼠心室肌细胞(NRVM)
  • 在人间充质干细胞(hMSC)/样品中，进行相对碱性磷酸酶(ALP)量标准化
  • 小鼠胚胎成纤维细胞（NIH3T3细胞）

特点和优势

  • 专用于ng级别DNA的定量
  • 适用于纯化制备物，和纯化含有RNA和蛋白质的粗提取物中的DNA

仅试剂盒组分

产品编号
说明
  • Detailed technical bulletin 1 ea

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. I see that one of the componets of Product DNAQF, DNA Quantitation Kit, Fluorescence Assay, is a DNA standard. How is the DNA standard prepared? Can it be purchased separately?  

    The DNA standard (Product No. D4810) used in the kit is a sheared calf thymus DNA. It is the only one tested with the kit and it is only available in the kit. We do not determine the molecular weight.

  6. How can I ensure accurate initial quantification of the DNA standard by spectrophotometric measurement at A260 when using Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?

    The DNA standard (Product No. D4810) should be incubated at 50 °C for 15-30 minutes before determining the concentration spectrophotometrically. This will ensure complete dissolution of the genomic DNA.

  7. What quality of genomic DNA is required for use in Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?

    Genomic DNA purified by phenol chloroform extraction is sufficiently clean for use with this kit.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Brian D Fink et al.
PloS one, 7(6), e39430-e39430 (2012-06-30)
It has been suggested that cells that are independent of insulin for glucose uptake, when exposed to high glucose or other nutrient concentrations, manifest enhanced mitochondrial substrate oxidation with consequent enhanced potential and generation of reactive oxygen species (ROS); a
Kausik Kapat et al.
ACS applied materials & interfaces, 9(45), 39235-39248 (2017-10-24)
Cementless fixation for orthopedic implants aims to obviate challenges associated with bone cement, providing long-term stability of bone prostheses after implantation. The application of porous titanium and its alloy-based implants is emerging for load-bearing applications due to their high specific
The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware.
Weiszenstein M
PLoS ONE, 11(3), e0152382-e0152382 (2016)
J M Teare et al.
BioTechniques, 22(6), 1170-1174 (1997-06-01)
Molecular biology is now a routine tool in almost all biological research fields. With the exponential growth in the number of molecular biological techniques, there is a recognizable need for sensitive, accurate and precise quantitation of nucleic acids. We present
Claire G Jeong et al.
Journal of biomedical materials research. Part A, 100(8), 2088-2096 (2012-05-23)
As articular cartilage is avascular, and mature chondrocytes do not proliferate, cartilage lesions have a limited capacity for regeneration after severe damage. The treatment of such damage has been challenging due to the limited availability of autologous healthy cartilage and
查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门