质量水平
形式
liquid
用途
1000 uses (0.5 mg size)
200 uses (0.1 mg size)
适用性
suitable for electrophoresis (DNA)
储存温度
−20°C
相关类别
一般描述
Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
应用
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
组分
10mM Tris-HCl 中的溶液，pH 8.0，1mM EDTA
其他说明
Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
制备说明
Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
商品
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
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