产品名称
pBR322 Hae III Digest, for DNA electrophoresis
form
liquid
usage
1000 uses (0.5 mg size)
200 uses (0.1 mg size)
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
Quality Level
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
General description
Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Other Notes
Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Preparation Note
Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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商品
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
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