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Merck
CN

D9538

Sigma-Aldrich

N,N′-(1,2-Dihydroxyethylene)bis-acrylamide

suitable for electrophoresis

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别名:
DHEBA
经验公式(希尔记法):
C8H12N2O4
CAS号:
分子量:
200.19
Beilstein:
1941875
EC 号:
MDL编号:
UNSPSC代码:
12162002

技术

electrophoresis: suitable

电导率

<60 μmho (2% solution)

储存温度

2-8°C

SMILES字符串

OC(NC(=O)C=C)C(O)NC(=O)C=C

InChI

1S/C8H12N2O4/c1-3-5(11)9-7(13)8(14)10-6(12)4-2/h3-4,7-8,13-14H,1-2H2,(H,9,11)(H,10,12)

InChI key

ZMLXKXHICXTSDM-UHFFFAOYSA-N

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应用

作为聚丙烯酰胺凝胶的可逆交联剂,能够生成凝胶,该凝胶于室温下在高碘酸稀释溶液 (2%) 中 1-2 小时可溶解。凝胶增溶对于从聚丙烯酰胺凝胶中回收蛋白质非常有用。

分析说明

经过测试可用作交联剂,用于回收分离的生物分子。

象形图

Exclamation mark

警示用语:

Warning

危险分类

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

法规信息

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A method for the quantitative determination of protein incorporated in solubilized polyacrylamide gels.
J Tas et al.
Analytical biochemistry, 100(2), 264-270 (1979-12-01)
U Neumann et al.
Analytical biochemistry, 206(1), 1-5 (1992-10-01)
A simple and rapid method for the quantitation of proteins separated either by sodium dodecyl sulfate-electrophoresis or by isoelectric focusing in slab gels is presented. The method is based on the solubility of polyacrylamide gels crosslinked with N, N'-1, 2-dihydroxyethylenebisacrylamide
Polyacrylamide gels with modified cross-linkages.
P B O'Connell et al.
Analytical biochemistry, 76(l), 63-73 (1976-11-01)
M Burow et al.
Biochemical and biophysical research communications, 227(2), 419-422 (1996-10-14)
A phosphate buffer containing a mixture of glucose oxidase, acrylic acid derivatives, N,N'-1,2 dihydroxy-ethylene-bis(acrylamide), N,N'-(methylene)-bisacrylamide and surface-modified silica was radically polymerized with (NH4)2S2O8. The polymer formed a thin layer around the silica beads. After sieving of these polymer particles, the
M Galvani et al.
Electrophoresis, 21(17), 3684-3692 (2001-03-29)
A number of cross-linkers that are commonly used in polyacrylamide gels have been incubated with bovine beta-lactoglobulin B and the resulting reaction mixtures were examined by matrix assisted laser desorption/ionization-mass spectrometry. At concentrations of 0.1, 1, and 20 mM of

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