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安全信息

D9281

Sigma-Aldrich

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

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About This Item

UNSPSC代码:
41141827
NACRES:
NA.25

质量水平

100
200

形式

liquid

用途

100 uses (0.1 mg size)
500 uses (0.5 mg size)

适用性

suitable for electrophoresis (DNA)

储存温度

−20°C

一般描述

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

应用

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

组分

10mM Tris-HCl 中的溶液,pH 8.0,1mM EDTA

其他说明

Contains 13 fragments, 125–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.

制备说明

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Sens. 1

储存分类代码

13 - Non Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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MOLECULAR CHARACTERIZATION OF FIVE SELECTED BRINJAL (Solanum melongena L) GENOTYPES USING SSR MARKERS
Khorsheduzzaman A K M, et al.
Bangladesh Journal of Plant Breeding and Genetics, 21(1), 01-06 (2008)

商品

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

实验方案

GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

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