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Merck
CN

D9170

Sigma-Aldrich

二甲基亚砜

PCR Reagent

别名:

DMSO, 甲基亚砜

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About This Item

线性分子式:
(CH3)2SO
CAS号:
分子量:
78.13
Beilstein:
506008
EC 号:
MDL编号:
UNSPSC代码:
12191502
PubChem化学物质编号:
NACRES:
NA.25

等级

PCR Reagent

质量水平

蒸汽密度

2.7 (vs air)

蒸汽压

0.42 mmHg ( 20 °C)

检测方案

≥99.5%

形式

liquid

自燃温度

573 °F

保质期

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

包装

vial of 1 mL

技术

PCR: suitable

杂质

≤0.1% water
water

颜色

colorless

折射率

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

溶解性

H2O: miscible (completely)

密度

1.10 g/mL (lit.)

适用性

suitable for PCR

应用

agriculture

SMILES字符串

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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一般描述

二甲基亚砜是自由基清除剂和超低温保存剂。其在双链断裂(DSB)中起保护作用。二甲基亚砜(1-10%)已显示出可加速链复性,并被认为可使核酸对热稳定,抗脱嘌呤。作为PCR助溶剂,DMSO或能有助于提高产量,尤其对长PCR,这一作用更加明显。

应用

二甲基亚砜用作聚合酶链反应(PCR)混合物的组分和 PCR 促进剂。

注意

在室温下容易过冷并缓慢重融。 固化产品可以通过加热至室温来重新液化,而不会损害产品。

其他说明

有关二甲基亚砜混溶性的信息,请访问以下链接:
二甲基亚砜混溶性/不混溶性表

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

  1. What is the molarity of DMSO?

    DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  2. What is the DMSO dissolved in?

    DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

  3. Where does DMSO come from?

    DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

  4. Does DMSO have any synthetic utility?

    DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
Ravikumar et al.
Applied Biochemistry and Biotechnology, 175(1), 3856-3862 (2015)
Microtitre Plate Based Cell-SELEX Method
Shorie M and Kaur H
Bio-protocol, 8(20) (2018)
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and gamma-ray-irradiation, and freezing
Noda M, et al.
Scientific reports, 7(1), 8557-8557 (2017)
IRAP and REMAP for retrotransposon-based genotyping and fingerprinting
Kalendar R and Schulman AH
Nature Protocols, 1(5), 2478-2478 (2006)
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, Todd C
Journal of Visualized Experiments, (63) (2012)

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