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Merck
CN

D9163

Sigma-Aldrich

DL-二硫苏糖醇

suitable for electrophoresis, ≥99%

别名:

1,4-二巯基苏糖醇, DTT, 克莱兰氏试剂

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About This Item

线性分子式:
HSCH2CH(OH)CH(OH)CH2SH
CAS号:
分子量:
154.25
Beilstein:
1719757
EC 号:
MDL编号:
UNSPSC代码:
12161504
PubChem化学物质编号:
NACRES:
NB.22

质量水平

检测方案

≥99%

形式

powder

反应适用性

reagent type: reductant

技术

electrophoresis: suitable

mp

40-44 °C
41-44 °C (lit.)

溶解性

H2O: 50 mg/mL, clear, colorless to very faintly yellow

痕量阳离子

heavy metals (as Pb): ≤10 ppm

吸光度

≤0.04 at 280 at 0.02 M

储存温度

2-8°C

SMILES字符串

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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应用

用于将 −SH 基保持在还原态的绝佳试剂;定量还原二硫化物。在 SDS-PAGE 前,DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常,使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。

分析说明

针对用于还原 SDS-PAGE 进行了测试

象形图

CorrosionExclamation mark

警示用语:

Danger

危险声明

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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