D8940
Anti-Destrin/ADF (AE-14) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
别名:
Anti-Actin Depolymerizing Factor
产品名称
Anti-Destrin/ADF (AE-14) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse, rat, human
concentration
~0.8-1.2 mg/mL
technique(s)
microarray: suitable
western blot: 1:600 using whole extracts of human A431 epidermoid carcinoma cells, rat PC-12 pheochromocytoma cells, or mouse NIH3T3 fibroblasts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DSTN(11034)
mouse ... Dstn(56431)
rat ... LOC296197(296197)
Application
Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:600 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and mouse NIH/3T3 fibroblasts, and a chemiluminescent detection reagent.
Biochem/physiol Actions
Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca2+ independent manner. It intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. It is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, and cytokinesis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Destrin/ADF and cofilin are widely distributed in tissues of eukaryotes and both contain a nuclear localization sequence. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. This gene is located on human chromosome 20p12.
Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in ‘Hirano bodies′ in certain brain neurons of dementia patients.
Immunogen
synthetic peptide corresponding to amino acids 137-150 of human destrin/ADF.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
The ADF/cofilin family: actin-remodeling proteins
Maciver SK and Hussey PJ
Genome Biology, 3(5), reviews3007-reviews3001 (2002)
Genome-wide association study of antiphospholipid antibodies
Kamboh MI, et al.
Autoimmune diseases, 2013 (2013)
W Rozek et al.
Polish journal of veterinary sciences, 16(4), 663-669 (2013-01-01)
Changes in the level of cellular proteins in cells inoculated with equine influenza virus H7N7 and H3N8 were studied with microarray technique. H3N8 induced pro-apoptotic proteins while H7N7 induced both pro- as well as anti-apoptotic factors. The higher level of
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